These mice express a codon improved Cre-recombinase in conventional dendritic cell precursors and may be useful for dendritic cell lineage fate mapping.
Caetano Reis e Sousa, London Research Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Clec9a | C-type lectin domain family 9, member a |
The coding region of the C-type lectin domain family 9, member a (Clec9a) gene is replaced by a codon improved version of Cre-recombinase (iCre), abolishing gene expression. Clec9a encodes for DNGR-1, an endocytic receptor of the C-type lectin superfamily that is selectively expressed at high levels by CD8α+ dendritic cells (DC) and at lower levels by plasmacytoid DC. DNGR-1 recognizes an intracellular ligand that is exposed in dead cells and facilitates cross-presentation of dead-cell-associated antigens through a mechanism that involves interaction of its intracellular tail with the tyrosine kinase, SYK. Homozygous mice are viable and fertile. When Clec9acre mice are bred with mice containing loxP-flanked sequence, iCre-mediated recombination will result in deletion of the floxed sequences in conventional DC precursors of the offspring.
A targeting vector was designed to replace the C-type lectin domain family 9, member a (Clec9a) coding region with a codon improved version of Cre-recombinase (iCre), a polyadenylation signal, and a loxP-flanked neomycin (neo) resistance cassette. This construct was electroporated into C57BL/6NTac-derived PRX-B6N embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females. Mutant mice were bred with B6.C-Tg(Pgk-cre)1Lni mice to remove the neo selection cassette. These Clec9acre mice were subsequently bred to C57BL/6J mice for at least 8 generations. Upon arrival at The Jackson Laboratory Repository, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre-recombinase is expressed in conventional dendritic cell precursors. |
Allele Name | targeted mutation 2.1, Caetano Reis e Sousa |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Clec9acre |
Gene Symbol and Name | Clec9a, C-type lectin domain family 9, member a |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre-recombinase is expressed in conventional dendritic cell precursors. |
Strain of Origin | C57BL/6NTac |
Chromosome | 6 |
Molecular Note | An existing targeting vector was modified by inserting a codon-improved cre sequence in place of the EGFP sequence in the pFloxRI-C9a-EGFP construct. This construct was electroporated into PRX-B6N (Primogenix) for homologous recombination. Animals carrying the cre allele were crossed to PGK-cre mice to delete the floxed neomycin selection cassette. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6J.B6N(Cg)-Clec9atm2.1(icre)Crs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025523 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Clec9a<tm2.1(icre)Crs> |
Frozen Mouse Embryo | B6J.B6N(Cg)-Clec9a<tm2.1(icre)Crs>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6J.B6N(Cg)-Clec9a<tm2.1(icre)Crs>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6J.B6N(Cg)-Clec9a<tm2.1(icre)Crs>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6J.B6N(Cg)-Clec9a<tm2.1(icre)Crs>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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