Overview

These mice express Cre-ER^T2 under the direction of the T promoter amaking them useful for applications requiring tamoxifen-induced deletion of floxed sequences during axis extension and somitogenesis.

Donating Investigator

Mark Lewandoski, National Cancer Institute

Genetic overview

Genetic Background	Generation

Tg(T-cre/ERT2)1Lwd

Allele Type
Transgenic (Recombinase-expressing, Inducible)

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Research Applications

Research Tools
Developmental Biology Research

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Expression in this Cre-ER^T2 transgenic line is directed by the T (brachyury) promoter/enhancer regions. T encodes a nuclear transcription factor active in progenitor cells that reside within the primitive streak and tail bud, and lineages that emerge from these tissues as the embryonic axis extends. This protein is essential to axis extension and somitogenesis. These mice express Cre-ER^T2 protein in all cells emerging from the primitive streak or tail bud throughout development. Restricted to the cytoplasm, Cre-ER^T2 can only gain access to the nuclear compartment after exposure to tamoxifen. Mice homozygous for the TCreERT2 transgene are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring.

For example, when bred to mice carrying Gt(ROSA)26Sor^tm1Sor (see Stock No. 003474), 24 hours after tamoxifen induction, widespread βgal activity is seen only in tissues recently emerged from the primitive streak at E7.5 and E8.5 or tailbud at E9.5–13.5. 48 hours after induction, TCreERT2;R26R embryos from E9.5–E12.5 display βgal activity that extends more rostrally.

When bred with B6.129-Ctnnb1^tm2Kem/KnwJ (Stock No. 003474), E8.5 and E10.5 embryos show disruption of somite patterning 48 after tamoxifen administration.

Development

A transgenic vector was generated including 600 bp of the T (brachyury) promoter driving expression of a creER^T2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain). This transgene was microinjected into fertilized (C57BL/6NCr X C3H/HeNcr) F2 oocytes. A male from one founder line was bred to a B6C3F1 female, and subsequently to other mutant lines. Contaminating alleles have been bred out of this line, and it has been maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.

Expression Data

Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Cre-ERT2 protein is expressed in all cells emerging from the primitive streak or tail bud throughout development.

Control Suggestions

Noncarrier ()

Additional Information

Considerations for Choosing Controls

Selected References

Anderson MJ; Naiche LA; Wilson CP; Elder C; Swing DA; Lewandoski M. 2013. TCreERT2, a transgenic mouse line for temporal control of Cre-mediated recombination in lineages emerging from the primitive streak or tail bud. PLoS One 8(4):e62479PubMed: 23638095 MGI: J:200549

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Genetics

Tg(T-cre/ERT2)1Lwd

Allele Symbol: Tg(T-cre/ERT2)1Lwd

Allele Name	transgene insertion 1, Mark Lewandoski
Allele Type	Transgenic (Recombinase-expressing, Inducible)
Allele Synonym(s)	transgene insertion 1, Mark Lewandoski; Tg(T-cre/ERT2)1Lwd
Gene Symbol and Name	Tg(T-cre/ERT2)1Lwd, transgene insertion 1, Mark Lewandoski
Gene Synonym(s)
Promoter	T, brachyury, T-box transcription factor T, mouse, laboratory
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Cre-ERT2 protein is expressed in all cells emerging from the primitive streak or tail bud throughout development.
Strain of Origin	(C57BL/6NCr x C3H/HeNCr)F2
Chromosome	UN
General Note	Zygotes for injection were derived by intercrossing B6C3F1 (C57BL/6NCr X C3H/HeNCrMTV) mice.
Molecular Note	The cre recombinase coding region fused to the sequence for a mutated ligand-binding domain of the human estrogen receptor was inserted into a construct where it is under the control of the 500 bp brachyury (T) promoter. Eight transgenic lines were generated and one which showed extensive cre activity in presence of tamoxifen but little without tamoxifen induction.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression: Inducible
  - Cre Recombinase Expression: Germline/Embryonic Expression
- Developmental Biology Research
  - Cre-lox System
Developmental Biology Research
- Growth Defects
- Limb Patterning Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg(T-cre/ERT2)1Lwd/Tg(T-cre/ERT2)1Lwd
involves: C3H/HeNCr * C57BL/6NCr

normal phenotype

no abnormal phenotype detected
- homozygotes show no obvious defects in health or fertility

References

Anderson MJ; Naiche LA; Wilson CP; Elder C; Swing DA; Lewandoski M. 2013. TCreERT2, a transgenic mouse line for temporal control of Cre-mediated recombination in lineages emerging from the primitive streak or tail bud. PLoS One 8(4):e62479PubMed: 23638095 MGI: J:200549

Additional - Tg(T-cre/ERT2)1Lwd related

Technical Support

Contact Technical Support

Genotyping Protocols
- QPCR: Generic Cre Quantitative PCR
- High Resolution Melting: Tg(Msx2-cre)
- MELT: Tg(T-cre/ERT2)1Lwd-Alternate 1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Tg(T-cre/ERT2)1Lwd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #025520 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizyous or Non Carrier for Tg(T-cre/ERT2)1Lwd

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(T-cre/ERT2)1Lwd

Allele Symbol: Tg(T-cre/ERT2)1Lwd

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tg(T-cre/ERT2)1Lwd/Tg(T-cre/ERT2)1Lwdinvolves: C3H/HeNCr * C57BL/6NCr

normal phenotype

References

Additional - Tg(T-cre/ERT2)1Lwd related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Genotype: Tg(T-cre/ERT2)1Lwd/Tg(T-cre/ERT2)1Lwd
involves: C3H/HeNCr * C57BL/6NCr