C57BL/6-Mc4r^Pkcp/JtJ

Stock number: 025249
Product name: Porkchop
Research areas: Diabetes and Obesity Research

Description

Both heterozygous and homozygous Pkcp (Porkchop) mutant mice rapidly become obese without signs of diabetes.

Detailed description

The semidominant porkchop (Pkcp) allele of the Mc4r (melanocortin 4 receptor) gene is defined by an arginine to tryoptophan (R147W; AGG->TGG) mutation. By 60-80 days of age, both heterozygous and homozygous mice (males and females) are significantly heavier than wildtype littermates. Homozygotes develop the obese phenotype earlier and are more severely affected than the heterozygotes. Diabetes has not been observed. Obesity can interfere with the ability of homozygotes to effectively breed.

Development

The semidominant Pkcp mutation in the Mc4r gene was created using ENU mutagenesis techniques by the Center for Functional Genomics (CFG) at Northwestern University as part of their Neurogenomics Project. Arginine 147 was mutated to tryptophan (R147W; AGG->TGG). The mutation was created and maintained on a C57BL/6J genetic background by the donating laboratory.

Allele

Symbol: Mc4r^Pkcp
Name: pork chop
MGI accession ID: MGI:3603635
Generation method: Chemically induced (ENU)
Synonyms: PorkChop
Marker symbol: Mc4r
Marker name: melanocortin 4 receptor
Marker synonyms: BMIQ20; Fatboy; Pkcp
Chromosome: 18
Strain of origin: C57BL/6J
Molecular note: ENU mutagenesis induced an A to T point mutation that results in the amino acid substitution of tryptophan for arginine at position 147 (R147W).