These Lfngflox mutant mice possess loxP sites flanking exon 2 of the lunatic fringe (Lfng) gene, an N-acetylglucosamine transferase that acts in the Notch signaling pathway. This strain may be useful for studying lung alveogenesis, myofibroblast differentiation and basal-like breast cell cancers.
Sean E. Egan, Hospital for Sick Children
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Lfng | LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
These Lfngflox mutant mice possess loxP sites flanking exon 2 of the lunatic fringe (Lfng) gene, an N-acetylglucosamine transferase that acts in the Notch signaling pathway. Lfng is expressed in distal lung during saccular development and basal cells in the developing mammary gland. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. This strain may be useful for studying lung alveogenesis, myofibroblast differentiation and basal-like breast cell cancers.
When bred to mice carrying Tg(EIIa-cre)C5379Lmgd, cre-expression in the early embryo results in mice exhibiting defects in lung structure, defective alveolar septation and impaired myofibroblast differentiation.
When bred to mice carrying Tg(MMTV-cre)1Mam, cre-expression in the mammary gland results in mice exhibiting basal-like mammary tumors with accumulation of activated Notch intracellular domain polypeptides.
A targeting vector was designed to insert a loxP site upstream of exon 2 and a FRT-loxP-PGKneo-FRT sequence followed by a third loxP site downstream of exon 2. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and resulting chimeric mice were bred. Offspring were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J to delete the neo cassette. The resulting progeny were crossed to FVB/N for more than 10 generations. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
Allele Name | targeted mutation 1.1, Sean E Egan |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Lfngflox |
Gene Symbol and Name | Lfng, LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 5 |
Molecular Note | A loxP site was inserted upstream of exon 2 and a neo cassette flanked by loxP and FRT sites was inserted downstream of exon 2. Flp-mediated recombination removed the neo cassette leaving exon 2 floxed. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the FVB.129-Lfngtm1.1Egan/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37160 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.