Overview

Donating Investigator

Sean E. Egan, Hospital for Sick Children

Genetic overview

Genetic Background	Generation

Lfng^tm1.1Egan

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Lfng	LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

View Genetics

Research Applications

Cancer Research
Research Tools

View All Research Applications

Details

Detailed Description

These Lfng^flox mutant mice possess loxP sites flanking exon 2 of the lunatic fringe (Lfng) gene, an N-acetylglucosamine transferase that acts in the Notch signaling pathway. Lfng is expressed in distal lung during saccular development and basal cells in the developing mammary gland. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. This strain may be useful for studying lung alveogenesis, myofibroblast differentiation and basal-like breast cell cancers.

When bred to mice carrying Tg(EIIa-cre)C5379Lmgd, cre-expression in the early embryo results in mice exhibiting defects in lung structure, defective alveolar septation and impaired myofibroblast differentiation.

When bred to mice carrying Tg(MMTV-cre)1Mam, cre-expression in the mammary gland results in mice exhibiting basal-like mammary tumors with accumulation of activated Notch intracellular domain polypeptides.

Development

A targeting vector was designed to insert a loxP site upstream of exon 2 and a FRT-loxP-PGKneo-FRT sequence followed by a third loxP site downstream of exon 2. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and resulting chimeric mice were bred. Offspring were bred with 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym/J to delete the neo cassette. The resulting progeny were crossed to FVB/N for more than 10 generations. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.

Selected References

Xu K; Nieuwenhuis E; Cohen BL; Wang W; Canty AJ; Danska JS; Coultas L; Rossant J; Wu MY; Piscione TD; Nagy A; Gossler A; Hicks GG; Hui CC; Henkelman RM; Yu LX; Sled JG; Gridley T; Egan SE. 2010. Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis. Am J Physiol Lung Cell Mol Physiol 298(1):L45-56PubMed: 19897741 MGI: J:155893
Xu K; Usary J; Kousis PC; Prat A; Wang DY; Adams JR; Wang W; Loch AJ; Deng T; Zhao W; Cardiff RD; Yoon K; Gaiano N; Ling V; Beyene J; Zacksenhaus E; Gridley T; Leong WL; Guidos CJ; Perou CM; Egan SE. 2012. Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer. Cancer Cell 21(5):626-41PubMed: 22624713 MGI: J:189299

View All References

Genetics

Lfng^tm1.1Egan

Allele Symbol: Lfng^tm1.1Egan

Allele Name	targeted mutation 1.1, Sean E Egan
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Lfng^flox
Gene Symbol and Name	Lfng, LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene Synonym(s)
Strain of Origin	129
Chromosome	5
Molecular Note	A loxP site was inserted upstream of exon 2 and a neo cassette flanked by loxP and FRT sites was inserted downstream of exon 2. Flp-mediated recombination removed the neo cassette leaving exon 2 floxed.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Cancer Research
- Cre-lox System
  - loxP-flanked Sequences
Research Tools
- Cre-lox System
  - loxP-flanked Sequences
- Developmental Biology Research
  - Cre-lox System
- Cancer Research
  - Cre-lox System

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Lfng^tm1.1Egan/Lfng⁺
involves: 129 * 129S4/SvJaeSor

mammalian phenotype

no phenotypic analysis
- Normal - mice were used to generate Lfng^tm1.2Egan homozygotes

References

Xu K; Nieuwenhuis E; Cohen BL; Wang W; Canty AJ; Danska JS; Coultas L; Rossant J; Wu MY; Piscione TD; Nagy A; Gossler A; Hicks GG; Hui CC; Henkelman RM; Yu LX; Sled JG; Gridley T; Egan SE. 2010. Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis. Am J Physiol Lung Cell Mol Physiol 298(1):L45-56PubMed: 19897741 MGI: J:155893
Xu K; Usary J; Kousis PC; Prat A; Wang DY; Adams JR; Wang W; Loch AJ; Deng T; Zhao W; Cardiff RD; Yoon K; Gaiano N; Ling V; Beyene J; Zacksenhaus E; Gridley T; Leong WL; Guidos CJ; Perou CM; Egan SE. 2012. Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer. Cancer Cell 21(5):626-41PubMed: 22624713 MGI: J:189299

Additional - Lfng^tm1.1Egan related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Lfng
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the FVB.129-Lfng^tm1.1Egan/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37160 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Selected References

Lfngtm1.1Egan

Allele Symbol: Lfngtm1.1Egan

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Lfngtm1.1Egan/Lfng+involves: 129 * 129S4/SvJaeSor

mammalian phenotype

References

Additional - Lfngtm1.1Egan related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Lfng^tm1.1Egan

Allele Symbol: Lfng^tm1.1Egan

Genotype: Lfng^tm1.1Egan/Lfng⁺
involves: 129 * 129S4/SvJaeSor

Additional - Lfng^tm1.1Egan related