These Lfngflox mutant mice possess loxP sites flanking exon 2 of the lunatic fringe (Lfng) gene, an N-acetylglucosamine transferase that acts in the Notch signaling pathway. This strain may be useful for studying lung alveogenesis, myofibroblast differentiation and basal-like breast cell cancers.
Sean E. Egan, Hospital for Sick Children
These Lfngflox mutant mice possess loxP sites flanking exon 2 of the lunatic fringe (Lfng) gene, an N-acetylglucosamine transferase that acts in the Notch signaling pathway. Lfng is expressed in distal lung during saccular development and basal cells in the developing mammary gland. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. This strain may be useful for studying lung alveogenesis, myofibroblast differentiation and basal-like breast cell cancers.
When bred to mice carrying Tg(EIIa-cre)C5379Lmgd, cre-expression in the early embryo results in mice exhibiting defects in lung structure, defective alveolar septation and impaired myofibroblast differentiation.
When bred to mice carrying Tg(MMTV-cre)1Mam, cre-expression in the mammary gland results in mice exhibiting basal-like mammary tumors with accumulation of activated Notch intracellular domain polypeptides.
A targeting vector was designed to insert a loxP site upstream of exon 2 and a FRT-loxP-PGKneo-FRT sequence followed by a third loxP site downstream of exon 2. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and resulting chimeric mice were bred. Offspring were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J to delete the neo cassette. The resulting progeny were crossed to FVB/N for more than 10 generations. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1.1, Sean E Egan|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Lfng, LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase|
|Strain of Origin||129|
|Molecular Note||A loxP site was inserted upstream of exon 2 and a neo cassette flanked by loxP and FRT sites was inserted downstream of exon 2. Flp-mediated recombination removed the neo cassette leaving exon 2 floxed.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the FVB.129-Lfngtm1.1Egan/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37160 in your Materials and Methods section.