| Allele Name | targeted mutation 1, Andrew P McMahon |
| Allele Type | Targeted (Recombinase-expressing, Reporter, Inducible, Null/Knockout, RMCE-ready) |
| Allele Synonym(s) | Spp2G2aCE; Spp2tm1(GFP,cre/ERT2)Amc |
| Gene Symbol and Name | Spp2, secreted phosphoprotein 2 |
| Gene Synonym(s) | |
| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Expressed Gene | cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene, |
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| Site of Expression | When mice carrying this allele are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in proximal tubule cells in the embryonic and adult kidney. |
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| Strain of Origin | C57BL/6N-Atm1Brd |
| Chromosome | 1 |
| General Note | This exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-Atm1Brd-derived JM8A3.N1 knockout first reporter embryonic stem (ES) cells obtained from the European Conditional Mouse Mutagenesis (EUCOMM) consortium containing clone EPDO558_1_A04). Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred toC57BL/6J to establish a colony. |
| Molecular Note | The allele was generated by a dual recombinase-mediated cassette exchange protocol. The exchange vector contained (from 5' to 3') an frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) sequence, a viral T2A oligopeptide, and a cre/ERT2 sequence. A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector. |
