JAX Mouse Strain Datasheet - 025209

C57BL/6-Spp2^{tm1(cre/ERT2)Amc}/J

Stock No:: 025209

Cryo Recovery

Overview

The Spp2^G2aCE knock-in allele was designed to both abolish Spp2 gene expression and express EGFP and Cre-ER^T2 proteins from the Spp2 promoter in proximal tubule cells of the mouse kidney.

Donating Investigator

Andrew P McMahon, University of Southern California

Genetic overview

Genetic Background	Generation

Spp2^{tm1(cre/ERT2)Amc}

Allele Type	Gene Symbol	Gene Name
Targeted (Inducible, Null/Knockout, RMCE-ready, Recombinase-expressing, Reporter)	Spp2	secreted phosphoprotein 2

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Research Applications

Developmental Biology Research
Endocrine Deficiency Research
Internal/Organ Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

The Spp2^G2aCE knock-in allele was designed to both abolish secreted phosphoprotein 2 (Spp2) gene expression and express individual EGFP and Cre-ER^T2 proteins from the Spp2 promoter/enhancer elements. SPP2 encodes a serum phosphoprotein synthesized by the liver which is involved in bone growth, repair, and turnover. Heterozygous Spp2^G2aCE mice are viable and fertile. The donating investigator has not attempted to make this strain homozygous, and mice may still be segregating for the A^tm1Brd allele. Weak EGFP fluorescence is seen in proximal tubule cells of the mouse kidney and in liver cells at 19.5 days post coitum. Cre-ER^T2 gene activity is inducible and can be observed following tamoxifen administration. As such, when these mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in proximal tubule cells in the embryonic and adult kidney.

This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).

Development

An exchange vector was designed to be inserted downstream of exon 1 of the secreted phosphoprotein 2 (Spp2) gene using dual-recombinase mediated cassette exchange (dRMCE). Specifically, the exchange vector contained (from 5' to 3') a frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) sequence, a viral T2A oligopeptide, and a Cre-ER^T2 sequence (Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain). A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector.

This exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-A^tm1Brd-derived JM8A3.N1 knockout first reporter embryonic stem (ES) cells obtained from the European Conditional Mouse Mutagenesis (EUCOMM) consortium containing clone EPDO558_1_A04. Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyr^c-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. Mice may still be segregating for the A^tm1Brd allele.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	When mice carrying this allele are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in proximal tubule cells in the embryonic and adult kidney.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Spp2^{tm1(cre/ERT2)Amc}

Allele Symbol: Spp2^{tm1(cre/ERT2)Amc}

Allele Name	targeted mutation 1, Andrew P McMahon
Allele Type	Targeted (Inducible, Null/Knockout, RMCE-ready, Recombinase-expressing, Reporter)
Allele Synonym(s)	Spp2^G2aCE; Spp2^{tm1(GFP,cre/ERT2)Amc}
Gene Symbol and Name	Spp2, secreted phosphoprotein 2
Gene Synonym(s)	0610038O04Rik; 0610038O04Rik; RIKEN cDNA 0610038O04 gene; SPP-24; SPP24; spp24
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	When mice carrying this allele are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in proximal tubule cells in the embryonic and adult kidney.
Strain of Origin	C57BL/6N-A
Chromosome	1
General Note	This exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-Atm1Brd-derived JM8A3.N1 knockout first reporter embryonic stem (ES) cells obtained from the European Conditional Mouse Mutagenesis (EUCOMM) consortium containing clone EPDO558_1_A04). Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred toC57BL/6J to establish a colony.
Molecular Note	The allele was generated by a dual recombinase-mediated cassette exchange protocol. The exchange vector contained (from 5' to 3') an frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) sequence, a viral T2A oligopeptide, and a cre/ERT2 sequence. A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Internal/Organ Defects
  - kidney
Endocrine Deficiency Research
- Kidney Defects
Internal/Organ Research
- Kidney Defects
Research Tools
- Cre-lox System
  - Cre Recombinase Expression: Inducible
- Fluorescent Proteins
- Genetics Research
  - Tissue/Cell Markers: hepatocytes
  - Tissue/Cell Markers: kidney specific marker

Genotype: GFP related

Research Tools
- Fluorescent Proteins

Mammalian Phenotype Terms by Genotype

Genotype: Spp2^{tm1(cre/ERT2)Amc}/Spp2⁺
C57BL/6-Spp2^{tm1(cre/ERT2)Amc}

no phenotypic analysis

no phenotypic analysis

References

Additional - Spp2^{tm1(cre/ERT2)Amc} related

McMahon A; Zhang P and colleagues, The GenitoUrinary Development Molecular Anatomy Project (GUDMAP). 2010. Summary of mouse strains characterized by GUDMAP consortium Unpublished :. MGI: J:159099

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wildtype for Spp2<tm1(GFP,cre/ERT2)Amc>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Spp2tm1(cre/ERT2)Amc

Allele Symbol: Spp2tm1(cre/ERT2)Amc

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: GFP related

Mammalian Phenotype Terms by Genotype

Genotype: Spp2tm1(cre/ERT2)Amc/Spp2+C57BL/6-Spp2tm1(cre/ERT2)Amc

no phenotypic analysis

References

Additional - Spp2tm1(cre/ERT2)Amc related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Spp2^{tm1(cre/ERT2)Amc}

Allele Symbol: Spp2^{tm1(cre/ERT2)Amc}

Genotype: Spp2^{tm1(cre/ERT2)Amc}/Spp2⁺
C57BL/6-Spp2^{tm1(cre/ERT2)Amc}

Additional - Spp2^{tm1(cre/ERT2)Amc} related