IWSR1/PdxJ

Stock number: 025206
Research areas: Neurobiology Research

Description

iWSR-1 mice were selectively bred to exhibit low alcohol withdrawal convulsion severity.

Detailed description

Inbred Withdrawal Seizure-Resistant, replicate 1 (iWSR-1) mice were inbred for 20 generations from a selected line of mice developed for low alcohol withdrawal seizure severity. For the selection, offspring from first litters were made physically dependent on ethanol and were then tested for ethanol withdrawal severity. The Withdrawal Seizure-Resistant replicate lines were selectively bred for high alcohol withdrawal convulsion severity after 72hr of continuous chronic ethanol vapor inhalation. The inbred strain iWSR-1 exhibits seizures approximately 10-fold less severe after an identical regimen of chronic exposure to ethanol than the inbred Withdrawal Seizure-Prone, replicate 2 (iWSP-2) strain that was developed in tandem (Stock No. 025205).

Development

Inbred Withdrawal Seizure-Resistant, replicate 1 (iWSR-1) mice where inbred from a line of mice (WSR-1) selectively bred for low alcohol withdrawal convulsion severity after 72hr of continuous chronic ethanol vapor inhalation. The founder stock for selective breeding was the HS/Ibg heterogeneous stock that was developed by Gerald McClearn and colleagues (The Institute for Behavioral Genetics, Boulder, Colorado) as an 8-way inbred strain cross of the following inbred strains obtained from the Cancer Research Genetics Laboratory at UC-Berkeley: A, AKR, BALB/c, C3H/2, C57BL, DBA/2, Is/Bi, and RIII. Generation 29 mice were purchased, and generation 31 HS mice became the foundation (S_o) population for selection based on alcohol withdrawal severity. After the WSR-1 line had been selected for 26 generations, the inbred iWSR-1 strain mice were brother-sister mated for at least 20 generations, after which a rotational breeding scheme based on 9 families was used.