Overview

This TALEN-mediated deletion in Alb results in the absence of any albumin protein in the blood. When combined with the knockout allele of the FcRn a-chain and a transgene expressing a human FcRn a-chain (FCGRT) under control of the human FcRn promoter, this albumin-deficient model may be useful for studying human serum albumin pharmacokinetics.

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Donating Investigator

Michael Wiles, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
	N1F4+F11 (2019-06-12 00:00:00)

Fcgrt^tm1Dcr

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Fcgrt	Fc receptor, IgG, alpha chain transporter

Tg(FCGRT)32Dcr

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

Alb^em12Mvw

Allele Type	Gene Symbol	Gene Name
Endonuclease-mediated (Null/Knockout)	Alb	albumin

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Metabolism Research
Research Tools

View All Research Applications

Base Price

Starting at:

$425.00 Domestic price for female 4-week

550.00 Domestic price for breeder pair

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Details

Detailed Description

Mice homozygous for the Alb^em12Mvw allele lack albumin protein in their blood. FcRn-/- hFcRn (32) Tg mice harbor a knockout allele of the Fc receptor, IgG, alpha chain transporter (Fcgrt^tm1Dcr) and express a human FcRn a-chain (FCGRT) transgene under control of the human FcRn promoter. Mouse IgG levels are low due to the species-specific activity of human FcRn. Mice do not exhibit any gross abnormal phenotype. This albumin-deficient model may be useful for studying the biology and pathobiology of serum albumin as well as evaluating human albumin pharmacokinetics.

C57BL/6J-Alb^em8Mvw/MvwJ (Stock No. 025200) was generated as a control for this strain.

Development

A targeting scheme was designed using a TALEN-mediated Non-Homologous End Joining (NHEJ) repair pathway to knock out the mouse albumin gene in B6.Cg-Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/DcrJ ((Stock No. 014565)). TALEN mRNAs were introduced into mutant zygotes, and the resulting founder mouse (#6074) was crossed to a B6.Cg-Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/DcrJ mouse in order to characterize the mutation, a 2-base deletion resulting in early termination of the translated protein. N1 Heterozygous offspring were filial-crossed to establish the colony as homozygotes.

Expression Data

Expressed Gene	FCGRT, Fc fragment of IgG receptor and transporter, human
Site of Expression
Site of Expression

Control Suggestions

Approximate Controls

Depending upon the nature of the experiment, the following mouse line(s) may be appropriate approximate controls:
-- Stock No. 025200
-- Stock No. 014565
000664 C57BL/6J
003982 B6.129X1-Fcgrt^tm1Dcr/DcrJ

Additional Information

Considerations for Choosing Controls

Selected References

Roopenian DC; Low BE; Christianson GJ; Proetzel G; Sproule TJ; Wiles MV. 2015. Albumin-deficient mouse models for studying metabolism of human albumin and pharmacokinetics of albumin-based drugs. MAbs 7(2):344-51PubMed: 25654695 MGI: J:220803

View All References

Genetics

Fcgrt^tm1Dcr

Allele Symbol: Fcgrt^tm1Dcr

Allele Name	targeted mutation 1, Derry C Roopenian
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	FcRn^-
Gene Symbol and Name	Fcgrt, Fc receptor, IgG, alpha chain transporter
Gene Synonym(s)
Strain of Origin	129X1/SvJ
Chromosome	7
Molecular Note	Sequence from exon 1 and part of exon2 was replaced with a PGK-neo cassette. Quantitative PCR of liver cDNA indicated the absence of mRNA. Western blot analysis of neonatal intestinal extracts failed to reveal protein product.

Tg(FCGRT)32Dcr

Allele Symbol: Tg(FCGRT)32Dcr

Allele Name	transgene insertion 32, Derry C Roopenian
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	hFcRn(32) vhFcRn (32) Tg hFcRn (32) Tg; Tg32
Gene Symbol and Name	Tg(FCGRT)32Dcr, transgene insertion 32, Derry C Roopenian
Gene Synonym(s)
Promoter	FCGRT, Fc fragment of IgG receptor and transporter, human
Expressed Gene	FCGRT, Fc fragment of IgG receptor and transporter, human
Strain of Origin	C57BL/6J
Chromosome	2
Molecular Note	A 34Kb fragment of a BAC containing the entire human FCGRT , Fc fragment of IgG, receptor, transporter, alpha, gene (approximately 11kb) and approximately 10kb of flanking sequence was sublconed into a SuperCos vector. The resulting cosmid was microinjected into fertilized C57BL/6J mouse eggs. Founder line 32 was established. Transgene insertion occurred on Chr 2.

Alb^em12Mvw

Allele Symbol: Alb^em12Mvw

Allele Name	endonuclease-mediated mutation 12, Michael Wiles
Allele Type	Endonuclease-mediated (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Alb, albumin
Gene Synonym(s)
Strain of Origin	B6.Cg-Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/DcrJ
Chromosome	5
Molecular Note	Exon 4 was targeted by introduction of TALEN mRNA into fertilized B6.Cg-Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/DcrJ oocytes and the resulting founder screened and found to have a 2 base pair deletion beginning at Chromosome 5 base 90,464,113 (GRCm38.p2) which is predicted to result in a frame shift then a premature stop codon 5 amino acids downstream of the encoded frame shift. By ELISA, heterozgyotes have a decrease in serum albumin of approximately 30 percent and homozygotes have no detectable serum albumin.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Fcgrt^tm1Dcr related

Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
- Immunodeficiency
  - defects in humoral immune responses
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - genes regulating susceptibility to infectious disease and endotoxin
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - genes regulating susceptibility to infectious disease and endotoxin

Metabolism Research

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Alb^em12Mvw/Alb^em12Mvw Fcgrt^tm1Dcr/Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/Tg(FCGRT)32Dcr
B6.Cg-Tg(FCGRT)32Dcr Alb Fcgrt/MvwJ

homeostasis/metabolism phenotype

decreased circulating serum albumin level
- mice lack albumin protein in their blood

References

Roopenian DC; Low BE; Christianson GJ; Proetzel G; Sproule TJ; Wiles MV. 2015. Albumin-deficient mouse models for studying metabolism of human albumin and pharmacokinetics of albumin-based drugs. MAbs 7(2):344-51PubMed: 25654695 MGI: J:220803

Additional - Alb^em12Mvw related

Additional - Fcgrt^tm1Dcr related

Additional - Tg(FCGRT)32Dcr related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- End Point Analysis:Alb-EP
- Standard PCR:Fcgrt
- Standard PCR:Fcgrt Alternate1
- QPCR:Tg(FCGRT)32Dcr
- Probe:Tg(FCGRT)-Chr2-Probe-alternate1
- Standard PCR:Fcgrttm1Dcr Alternate1
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K20 formulation (10% fat)
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes. Experience at The Jackson Laboratory indicates mating males and females homozygous for all 3 genes to be highly non-productive and litters produced often don't make it to wean. However, we have find that mating het, hom, hom x hom, hom, hom and reciprocal to consistantly provide litters. It is not uncommon to lose the first litter.
- Additional Breeding and Husbandry Support
Mating System
- heterozygous for Alb^em12Mvw, Homozygous for Fcgrt^tm1Dcr, Homozygous for Tg(FCGRT)32Dcr x Homozygous for Alb^em12Mvw, Homozygous for Fcgrt^tm1Dcr, Homozygous for Tg(FCGRT)32Dcr
  or
  homozygous for Alb^em12Mvw, Homozygous for Fcgrt^tm1Dcr, Homozygous for Tg(FCGRT)32Dcr x Heterozygous for Alb^em12Mvw, Homozygous for Fcgrt^tm1Dcr, Homozygous for Tg(FCGRT)32Dc
Citation
When using the B6.Cg-Tg(FCGRT)32Dcr Alb^em12Mvw Fcgrt^tm1Dcr/MvwJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #025201 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX12 (Maximum)

Pricing & Availability

Available Now

Domestic
International

Domestic Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

International Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous and homozygous for Alb<em12Mvw>, Homzygous for Fcgrt<tm1Dcr> and Tg(FCGRT)32Dcr

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

MICE ARE NOT AVAILABLE FOR DISTRIBUTION TO CONTRACT RESEARCH ORGANIZATIONS

Notice regarding distribution of this strain.

Use of MICE by companies or for-profit entities requires a no-fee JAX Leap License prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Fcgrttm1Dcr

Allele Symbol: Fcgrttm1Dcr

Tg(FCGRT)32Dcr

Allele Symbol: Tg(FCGRT)32Dcr

Albem12Mvw

Allele Symbol: Albem12Mvw

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Fcgrttm1Dcr related

Mammalian Phenotype Terms by Genotype

Genotype: Albem12Mvw/Albem12Mvw Fcgrttm1Dcr/Fcgrttm1Dcr Tg(FCGRT)32Dcr/Tg(FCGRT)32DcrB6.Cg-Tg(FCGRT)32Dcr Alb Fcgrt/MvwJ

homeostasis/metabolism phenotype

References

Additional - Albem12Mvw related

Additional - Fcgrttm1Dcr related

Additional - Tg(FCGRT)32Dcr related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Fcgrt^tm1Dcr

Allele Symbol: Fcgrt^tm1Dcr

Alb^em12Mvw

Allele Symbol: Alb^em12Mvw

Genotype: Fcgrt^tm1Dcr related

Genotype: Alb^em12Mvw/Alb^em12Mvw Fcgrt^tm1Dcr/Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/Tg(FCGRT)32Dcr
B6.Cg-Tg(FCGRT)32Dcr Alb Fcgrt/MvwJ

Additional - Alb^em12Mvw related

Additional - Fcgrt^tm1Dcr related