The Crebbp gene encodes the CREB binding protein and the Ep300 gene encodes the E1A binding protein p300, both of which are highly conserved coactivators that promote transcription and are important to the function of many hematopoietic transcription factors. These mice possess loxP sites on either side of exon 9 of the targeted Crebbp gene. Exon 9 encodes amino acids 608-646 of the KIX domain. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 9 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in the mammary gland (see Stock No. 003553 for example), this mutant mouse strain may be useful in studies of T cell lymphoma.
When bred to a strain with Cre recombinase expression in T cells (see Stock No. 003802 for example), offspring exhibit decreased numbers of CD4+ T cells, an increase in double positive T cell and an increased incidence of T cell lymphomas.
