These CRE3 transgenic mice express Cre recombinase under the control of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter beginning at E11.5 in the brain. This strain may be useful for generating conditional deletions in neurons and their progenitors.
Stan Krajewski, Sanford/Burnham Medical Research Institute
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These CRE3 transgenic mice express Cre recombinase under the control of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter. Cre activity is observed beginning at E11.5 in the brain and only in cells that are committed to be neurons. Expression is found in the grey matter of the brain, cerebellum, spinal cord, retina, and dorsal and sympathetic ganglia. No expression is observed in the heart.
CRE3 mice may be useful for generating conditional deletions in neurons and their progenitors.
The transgenic construct contains Cre recombinase under the direction of a 250 bp fragment of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter. This transgene was microinjected into fertilized oocytes from a FVB/N female. Founder line 3 was established and maintained on an FVB/N background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is expressed in neurons in the brain. |
Allele Name | transgene insertion 3, Stan Krajewski |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | CRE3; MLC2v-CRE3; MLCcre |
Gene Symbol and Name | Tg(Mylpf-cre)3Kraj, transgene insertion 3, Stan Krajewski |
Gene Synonym(s) | |
Promoter | Mylpf, myosin light chain, phosphorylatable, fast skeletal muscle, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is expressed in neurons in the brain. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | A 250-bp fragment of the rat ventricular light chain II gene (Mylpf) was used as promoter to drive expression of the cre recombinase cDNA. Crosses of these mice were made to mice (dh4) carrying a transgenic reporter gene (a dominant-hygR gene separated from a constitutive PGK promoter by a loxP-flanked PGK-neo cassette; cre recombination results in generation of the active-hygR allele detected by PCR). Line 3 was determined to express cre selectively in the brain. |
While maintaining a live colony, these mice are bred as hemizygotes or homozygotes. The donating investigator indicates that males have a low spermatocyte count.
When using the MLC2v-CRE3 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37580 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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