Overview

Also Known As:MLC2v-CRE3

These CRE3 transgenic mice express Cre recombinase under the control of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter beginning at E11.5 in the brain. This strain may be useful for generating conditional deletions in neurons and their progenitors.

Donating Investigator

Stan Krajewski, Sanford/Burnham Medical Research Institute

Genetic overview

Genetic Background	Generation

Tg(Mylpf-cre)3Kraj

Allele Type
Transgenic (Recombinase-expressing)

View Genetics

Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Details

Detailed Description

These CRE3 transgenic mice express Cre recombinase under the control of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter. Cre activity is observed beginning at E11.5 in the brain and only in cells that are committed to be neurons. Expression is found in the grey matter of the brain, cerebellum, spinal cord, retina, and dorsal and sympathetic ganglia. No expression is observed in the heart.
CRE3 mice may be useful for generating conditional deletions in neurons and their progenitors.

Development

The transgenic construct contains Cre recombinase under the direction of a 250 bp fragment of the rat myosin light chain II ventricular (Mylpf or MLC2v) promoter. This transgene was microinjected into fertilized oocytes from a FVB/N female. Founder line 3 was established and maintained on an FVB/N background.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase is expressed in neurons in the brain.

Control Suggestions

Wild-type from the colony
001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Banares S; Zeh K; Krajewska M; Kermer P; Baribault H; Reed JC; Krajewski S. 2005. Novel pan-neuronal Cre-transgenic line for conditional ablation of genes in the nervous system. Genesis 42(1):6-16PubMed: 15828007 MGI: J:99342

View All References

Genetics

Tg(Mylpf-cre)3Kraj

Allele Symbol: Tg(Mylpf-cre)3Kraj

Allele Name	transgene insertion 3, Stan Krajewski
Allele Type	Transgenic (Recombinase-expressing)
Allele Synonym(s)	CRE3; MLC2v-CRE3; MLCcre
Gene Symbol and Name	Tg(Mylpf-cre)3Kraj, transgene insertion 3, Stan Krajewski
Gene Synonym(s)
Promoter	Mylpf, myosin light chain, phosphorylatable, fast skeletal muscle, rat
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase is expressed in neurons in the brain.
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	A 250-bp fragment of the rat ventricular light chain II gene (Mylpf) was used as promoter to drive expression of the cre recombinase cDNA. Crosses of these mice were made to mice (dh4) carrying a transgenic reporter gene (a dominant-hyg^R gene separated from a constitutive PGK promoter by a loxP-flanked PGK-neo cassette; cre recombination results in generation of the active-hyg^R allele detected by PCR). Line 3 was determined to express cre selectively in the brain.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue
Research Tools
- Cre-lox System
  - Cre Recombinase Expression: Germline/Embryonic Expression
  - Cre Recombinase Expression

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Tg(Mylpf-cre)3Kraj/Tg(Mylpf-cre)3Kraj
involves: FVB/N

normal phenotype

no abnormal phenotype detected
- Normal - homozygous mice are viable and fertile; no phenotypic description is provided

References

Banares S; Zeh K; Krajewska M; Kermer P; Baribault H; Reed JC; Krajewski S. 2005. Novel pan-neuronal Cre-transgenic line for conditional ablation of genes in the nervous system. Genesis 42(1):6-16PubMed: 15828007 MGI: J:99342

Additional - Tg(Mylpf-cre)3Kraj related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Generic Cre Probe
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as hemizygotes or homozygotes. The donating investigator indicates that males have a low spermatocyte count.
- Additional Breeding and Husbandry Support
Mating System
- Hemizygote x Noncarrier
- Noncarrier x Hemizygote
Citation
When using the MLC2v-CRE3 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37580 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:MLC2v-CRE3

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Mylpf-cre)3Kraj

Allele Symbol: Tg(Mylpf-cre)3Kraj

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tg(Mylpf-cre)3Kraj/Tg(Mylpf-cre)3Krajinvolves: FVB/N

normal phenotype

References

Additional - Tg(Mylpf-cre)3Kraj related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Genotype: Tg(Mylpf-cre)3Kraj/Tg(Mylpf-cre)3Kraj
involves: FVB/N