B6;D2-Tg(Fshr-cre)1Ldu/J

Stock number: 024926
Research areas: Developmental Biology Research, Endocrine Deficiency Research, Reproductive Biology Research, Research Tools

Description

These mice express Cre recombinase under the control of a truncated follicle stimulating hormone receptor (Fshr) promoter in ovarian granulosa cells and in cells from the anterior pituitary.

Detailed description

These mice express Cre recombinase under the control of a truncated follicle stimulating hormone receptor (Fshr) promoter. FSHR is a G-protein coupled receptor involved in gonad development. Hemizygotes are viable and fertile. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in ovarian granulosa cells and in a subset of cells from the anterior pituitary. FSHR promoter activity is also seen in mammary epithelium and in the olfactory bulb. The donating investigator maintains this colony by breeding wildtype females to hemizygous males to prevent germ line deletion since Cre is produced in ovarian granulosa cells.

When bred to a strain expressing a floxed-Brca1 gene, FSHR-deficient mice develop cysts on the ovary oviducts, and uterine horns; they also show increased estradiol biosynthesis and prolongation of the preovulatory phase (proestrus) of their estrus cycle resulting in increased estrogen exposure and ensuing consequences on estrogen responsive organs such as long bones.

Development

A transgenic construct containing cre coding sequence under the control of a truncated follicle stimulating hormone receptor (Fshr) promoter was introduced into B6D2F1 donor oocytes. Resulting offspring were bred together and were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Fshr-cre)1Ldu
Name: transgene insertion 1, Louis Dubeau
MGI accession ID: MGI:3575090
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Fshr-cre)1Ldu
Marker name: transgene insertion 1, Louis Dubeau
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase is expressed in ovarian granulosa cells and in cells from the anterior pituitary.
Molecular note: A transgenic construct containing cre coding sequence under the control of a truncated follicle stimulating hormone receptor (Fshr) promoter was introduced into B6D2F1 donor oocytes.