Exon 3 of the Sec24c gene is flanked by loxP sites in this conditional Sec24c-FL mutant strain. These mice are useful in studies of embryonic development and endoplasmic reticulem (ER)-Golgi vesicular trafficking.
David Ginsburg, University of Michigan
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed)) | Sec24c | Sec24 related gene family, member C (S. cerevisiae) |
SEC24 proteins are responsible for selectively recruiting cargo proteins from the endoplasmic reticulum (ER) into COPII vesicles that mediate transport to the Golgi. Sec24c (Sec24 related gene family, member C (S. cerevisiae)) is one of four mammalian Sec24 paralogs which are highly conserved at the C-terminus and variable in the N-terminal region. Mice completely lacking SEC24C die around embryonic day 7.
Exon 3 of the Sec24c gene is flanked by loxP sites in this conditional mutant strain. Heterozygous and homozygous Sec24c-FL mice are healthy, appear normal and are fertile. Cre excision of the floxed segment results in a frameshift mutation and early stop in exon 4, producing a null allele (see Stock No. 024868).
Progeny derived from crosses with a smooth muscle cell-specific Cre strain (SM22-Cre, see Stock No. 04746) do not have any grossly apparent abnormalities and are able to carry litters to term. Pups derived from crosses with albumin-Cre (hepatocyte-specific , see Stock No. 003574) and villin-cre (intestinal-epithelial-specific, see Stock No. 004586), show normal plasma cholesterol and triglyceride levels when on a high fat diet.
Embryonic stem (ES) cell clone EPD0241-2-A11 (EUCOMM ID: 32981) was created by the Wellcome Trust Sanger Institute as part of the Knockout Mouse Project (KOMP). The original gene trapped allele of Sec24c incorporated an FRT-En2 splice acceptor (SA)-IRES-lacZ-SV40 polyadenylation signal (pA)-loxP-PGK-Neo-SV40 polyadenylation signal (pA)-FRT-loxP sequence in intron 2 of the gene and an additional loxP site in intron 3 to create the Sec24c-GT (tm1a(EUCOMM)Wtsi) allele. C57BL/6N-derived JM8.N4 embryonic stem (ES) cells were used to create the mutation. Chimeras were crossed with B6(Cg)-Tyrc-2J/J (see Stock No. 000058) to achieve germline transmission. The lacZ and neomycin segments were excised with germline Flp recombinase (see Stock No. 005703), leaving exon 3 flanked by loxP sites. Mice were backcrossed to C57BL/6J for two generations to remove the FLP transgene. The Tyrc-2J allele was bred out of the line.
Allele Name | targeted mutation 1c, Wellcome Trust Sanger Institute |
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Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | Sec24cFL |
Gene Symbol and Name | Sec24c, Sec24 related gene family, member C (S. cerevisiae) |
Gene Synonym(s) | |
Strain of Origin | C57BL/6N |
Chromosome | 14 |
General Note | Cell line EPD0241_2_A11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:232065 |
Molecular Note | The L1L2_Pgk_P cassette was inserted at position 20679037 of Chromosome 14 upstream of exon 3. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 20679926. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, deleting the lacZ seqences and neomycin selection cassette, leaving exon 3 flanked by loxP sites. |
Heterozygotes and homozygotes are viable and fertile.
When using the B6(Cg)-Sec24ctm1c(EUCOMM)Wtsi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024867 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Sec24c<tm1c(EUCOMM)Wtsi> |
Frozen Mouse Embryo | B6(Cg)-Sec24c<tm1c(EUCOMM)Wtsi>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Sec24c<tm1c(EUCOMM)Wtsi>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Sec24c<tm1c(EUCOMM)Wtsi>/J | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Sec24c<tm1c(EUCOMM)Wtsi>/J | $3373.50 |
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