Overview

The deformed tail spontaneous mutation causes tail kinks and variable tail length, and likely causes prenatal lethality of approximately half of homozygotes.

Genetic overview

Genetic Background	Generation

dft

Allele Type	Gene Symbol	Gene Name
Spontaneous (Not Specified)	dft	deformed tail

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Research Applications

Developmental Biology Research
Internal/Organ Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Deformed tail homozygotes have tail kinks, with variability in the number of kinks and variable shortening of the tail length. In the most severe cases the tail is only a short stub. The overall size of the homozygote may be slightly smaller than its littermate controls. Prenatal lethality of some homozygotes is indicated by the smaller litter size and fewer than expected numbers of mutants generated in heterozygous intercrosses, which yielded an average of 13.3% mutants instead of 25%, or crosses between heterozgyotes and homozygotes, which yielded an average of 22.7% mutants instead of 50%. Preliminary mapping linked this mutation to distal Chromosome 5, with only 1 of 9 homozygotes being recombinant with rs3664741.

Development

The mutation deformed tail (dft) arose spontaneously and was identified in an F2 male from an F1 intercross of FVB/NJ with a C57BL/6J-Hr^rh-10J/GrsrJ homozygote. This male was bred to a C57BL/6J female and the line was maintained by sibling intercrossing thereafter. Sperm was cryopreserved from homozygous males at generation N1F2 and cryo-recovery is scheduled to use C57BL/6J oocytes.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Harris BS; Fairfield HE; Berry ML; Bronson RT; Reinholdt LG; Bergstrom DE. 2017. Deformed tail, a spontaneous mutation that maps to Chromosome 5 MGI Direct Data SubmissionMGI: J:243755

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Genetics

dft

Allele Symbol: dft

Allele Name	deformed tail
Allele Type	Spontaneous (Not Specified)
Allele Synonym(s)
Gene Symbol and Name	dft, deformed tail
Gene Synonym(s)
Strain of Origin	C57BL/6J-Hr^rh-10J/GrsrJ or FVB/NJ
Chromosome	5
General Note	The mutant phenotype was first observed "in an F2 male from an F1 intercross of FVB/NJ with a C57BL/6J-Hr^rh-10J/GrsrJ homozygote." (J:243755)
Molecular Note	This recessive mutation arose spontaneously at The Jackson Laboratory.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: dft related

Developmental Biology Research
- Skeletal Defects
- Embryonic Lethality (Homozygous)
  - incomplete
Internal/Organ Research
- Skeleton

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: dft/dft
B6;FVB-dft/GrsrJ

limbs/digits/tail phenotype

kinked tail

short tail
- variability in tail length, with the most severely impacted homozygote having only a short stub of a tail

mortality/aging

prenatal lethality, incomplete penetrance
- intercrossing heterozygotes with homozygotes yielded only 22.7% mutants instead of the expected 50% and intercrossing heterozygotes yielded only 13.3% mutants instead of the expected 25%, which combined with the lower than normal litter size indicates some loss of homozygotes to prenatal lethality

reproductive system phenotype

decreased litter size
- intercrosses of homozygotes with heterozygotes yielded an average litter size of only 4 and intercrosses of heterozygotes yielded an average litter size of approximately 5

References

Harris BS; Fairfield HE; Berry ML; Bronson RT; Reinholdt LG; Bergstrom DE. 2017. Deformed tail, a spontaneous mutation that maps to Chromosome 5 MGI Direct Data SubmissionMGI: J:243755

Additional - dft related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Mating System
- Progeny Tested
- heterozygote x heterozygot
- Homozygote x Heterozygote
- Heterozygote x Homozygote
Citation
When using the B6;FVB-dft/GrsrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #024840 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for dft

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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