B6;FVB-dft/GrsrJ

Stock number: 024840
Research areas: Developmental Biology Research, Internal/Organ Research

Description

The deformed tail spontaneous mutation causes tail kinks and variable tail length, and likely causes prenatal lethality of approximately half of homozygotes.

Detailed description

Deformed tail homozygotes have tail kinks, with variability in the number of kinks and variable shortening of the tail length. In the most severe cases the tail is only a short stub. The overall size of the homozygote may be slightly smaller than its littermate controls. Prenatal lethality of some homozygotes is indicated by the smaller litter size and fewer than expected numbers of mutants generated in heterozygous intercrosses, which yielded an average of 13.3% mutants instead of 25%, or crosses between heterozgyotes and homozygotes, which yielded an average of 22.7% mutants instead of 50%. Preliminary mapping linked this mutation to distal Chromosome 5, with only 1 of 9 homozygotes being recombinant with rs3664741.

Development

The mutation deformed tail (dft) arose spontaneously and was identified in an F2 male from an F1 intercross of FVB/NJ with a C57BL/6J-Hr^rh-10J/GrsrJ homozygote. This male was bred to a C57BL/6J female and the line was maintained by sibling intercrossing thereafter. Sperm was cryopreserved from homozygous males at generation N1F2 and cryo-recovery is scheduled to use C57BL/6J oocytes.

Allele

Symbol: dft
Name: deformed tail
MGI accession ID: MGI:5912231
Generation method: Spontaneous
Attributes: Not Specified
Marker symbol: dft
Marker name: deformed tail
Chromosome: 5
Strain of origin: C57BL/6J-Hr^rh-10J/GrsrJ or FVB/NJ
Molecular note: This recessive mutation arose spontaneously at The Jackson Laboratory.
General note: The mutant phenotype was first observed "in an F2 male from an F1 intercross of FVB/NJ with a C57BL/6J-Hr^rh-10J/GrsrJ homozygote." (J:243755)