C57BL/6J-Adamts20^bt-10J/GrsrJ

Stock number: 024803
Product name: belted 10 Jackson

Description

Belted mutants are valuable for characterizing the role of ADAMTS20 in melanoblast survival, palate development, inter-digital web regression and apoptosis involving the ADAMTS20 signaling pathway.

Detailed description

ADAMTS20 is a secreted metalloprotease. Mice homozygous for the belted 10 Jackson mutation display the typical white belt of the lower back on an otherwise black coat color. Although not characterized for this allele, the belt of other Adamts20 mutants was found result from the melanoblasts that migrate to this area failing to survive (Silver et al., 2008). Interdigital webbing was found when the Adamts20^bt-Bel1 belted homozygotes were compounded by heterozygosity for a mutant allele of Adamts9 or Adamts5 or versican or fibulin 1 (McCulloch et al., 2009).

Development

The belted 10 Jackson mutation arose spontaneously on a transgenic strain on pure C57BL/6J and the transgene was bred out of this mutant subline. Sperm was cryopreserved from homozygous males.

Allele

Symbol: Adamts20^bt-10J
Name: belted 10 Jackson
MGI accession ID: MGI:5617471
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Adamts20
Marker name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Chromosome: 15
Strain of origin: C57BL/6J
Molecular note: This spontaneous G to A single nucleotide variant at Chromosome 15 position 94,345,754 bp (GRCm38) is predicted to introduce a Q665* premature stop codon.