These Drd3 knockout mice exhibit altered responses to cocaine (D1 agonist) and amphetamine (D2 agonist) administered separately and together. This strain may be useful for studying dopamine neurotransmission.
Ming Xu, University of Chicago
Drd3 (dopamine receptor D3) is a G-protein coupled receptor expressed in the limbic system and involved in adenylyl cyclase inhibition. D3 is associated with motor and reward-related behavior. Mice homozygous for this knockout allele are viable and fertile. Drd3-deficient mice exhibit a transient increase in activity in a novel environment, increased activity at low, but not high, doses of cocaine, an altered sensitivity to amphetamine in the conditioned cue preference (CCP) test and enhanced behavioral sensitivity to a combined injection of cocaine and amphetamines (D1 and D2 agonists). This strain may be useful for studying dopamine neurotransmission
A targeting vector containing a PGKneo cassette was used to replace exon 1. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 and offspring were backcrossed to C57BL/6 for 2 generations. Upon arrival, mice were bred to C57BL/6J mice (Stock No. 000664) for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Susumu Tonegawa|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||D3-; D3R KO; D3R-|
|Gene Symbol and Name||Drd3, dopamine receptor D3|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A PGK-neo cassette replaced exon 1. Southern blot analysis confirmed recombination. Autoradiographic analysis confirmed the absence of Drd3 binding sites in brains of homozygous mice.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the D3R KO mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37114 in your Materials and Methods section.