B6;129S2-Drd3^tm1Stl/Mmjax

Stock number: 024766
Product name: D3R KO
Research areas: Neurobiology Research

Description

These Drd3 knockout mice exhibit altered responses to cocaine (D1 agonist) and amphetamine (D2 agonist) administered separately and together. This strain may be useful for studying dopamine neurotransmission.

Detailed description

Drd3 (dopamine receptor D3) is a G-protein coupled receptor expressed in the limbic system and involved in adenylyl cyclase inhibition. D3 is associated with motor and reward-related behavior. Mice homozygous for this knockout allele are viable and fertile. Drd3-deficient mice exhibit a transient increase in activity in a novel environment, increased activity at low, but not high, doses of cocaine, an altered sensitivity to amphetamine in the conditioned cue preference (CCP) test and enhanced behavioral sensitivity to a combined injection of cocaine and amphetamines (D1 and D2 agonists). This strain may be useful for studying dopamine neurotransmission

Development

A targeting vector containing a PGKneo cassette was used to replace exon 1. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 and offspring were backcrossed to C57BL/6 for 2 generations. Upon arrival, mice were bred to C57BL/6J mice (Stock No. 000664) for at least 1 generation to establish the colony.

Allele

Symbol: Drd3^tm1Stl
Name: targeted mutation 1, Susumu Tonegawa
MGI accession ID: MGI:3044114
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Drd3
Marker name: dopamine receptor D3
Chromosome: 16
Strain of origin: 129S2/SvPas
Molecular note: A PGK-neo cassette replaced exon 1. Southern blot analysis confirmed recombination. Autoradiographic analysis confirmed the absence of Drd3 binding sites in brains of homozygous mice.