These Drd1a knockout mice exhibit reduced body weight, increased locomotor activity and a loss of response to D1 agonists and antagonists. This strain may be useful for studying dopamine neurotransmission.
Ming Xu, University of Chicago
Drd1a (dopamine receptor D1A) is a G-protein coupled receptor involved in the regulation of neuronal growth and development. The D1 subtype is the most abundant dopamine receptor in the central nervous system. Defects in dopaminergic neurons are associated with Parkinson's disease, schizophrenia, attention-deficit and hyperactivity disorder, and compulsive drug abuse. Mice homozygous for this knockout allele are viable and fertile. Drd1a-deficient mice exhibit reduced body weight, increased locomotor activity, and a loss of response to cocaine administration as well as the stimulant and suppressive effects of D1 agonists and antagonists, respectively. This strain may be useful for studying dopamine neurotransmission.
A targeting construct with a PGK neomycin resistance cassette was designed to delete 95% of the coding sequence. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 and offspring were backcrossed to C57BL/6 for 2 generations. Upon arrival, mice were bred to C57BL/6J mice (Stock No. 000664) for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Susumu Tonegawa|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||D1-; D1R-|
|Gene Symbol and Name||Drd1, dopamine receptor D1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A PGK-neomycin based targeting vector replaced the 95% of the coding region.|
While maintaining a live colony, these mice can be bred as homozygotes, although the donating investigator indicates breeding is not as productive as in heterozygotes.
When using the D1R- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37113 in your Materials and Methods section.