Overview

Also Known As:Aqp9^-

These Aqp9 knockout mice exhibit increased levels of plasma glycerol and triglycerides and may be useful for studying the role of glycerol and glucose metabolism.

Donating Investigator

Aleksandra Rojek, Aarhus University

Genetic overview

Genetic Background	Generation

Aqp9^tm1Nlsn

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Aqp9	aquaporin 9

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Research Applications

Metabolism Research
Diabetes and Obesity Research

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Details

Detailed Description

Aquaporin 9 (Aqp9 ) encodes an aquaglyceroporin membrane channel known to conduct water, glycerol and other small solutes. Mice homozygous for this null allele exhibit increased levels of plasma glycerol and triglycerides.
This knockout may be useful for studying the role of glycerol and glucose metabolism.

Development

The targeting vector contains a PGK neomycin cassette that was used to replace 55 nucleotides in exon 2. The insertion results in correct translation of the first 47 amino acids followed by 100 random amino acids and a stop codon. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and backcrossed for at least 10 generations. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) mice for 1 generation to establish the colony.

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Rojek AM; Skowronski MT; Fuchtbauer EM; Fuchtbauer AC; Fenton RA; Agre P; Frokiaer J; Nielsen S. 2007. Defective glycerol metabolism in aquaporin 9 (AQP9) knockout mice. Proc Natl Acad Sci U S A 104(9):3609-14PubMed: 17360690 MGI: J:123742

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Genetics

Aqp9^tm1Nlsn

Allele Symbol: Aqp9^tm1Nlsn

Allele Name	targeted mutation 1, Soren Nielsen
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Aqp9^-
Gene Symbol and Name	Aqp9, aquaporin 9
Gene Synonym(s)
Strain of Origin	129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Chromosome	9
Molecular Note	A neomycin resistance gene replaced 55 nucleotides in exon 2. The insertion was predicted to result in correct translation of the initial 47 amino acids followed by 100 random amino acids and a stop codon. RT-PCR confirmed correct integration of the mutation. An aberrantly spliced transcript was detected, where exon 1 was fused to exon 3, however, the production of protein from this transcript is unlikely.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Metabolism Research
Diabetes and Obesity Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Aqp9^tm1Nlsn/Aqp9^tm1Nlsn
involves: 129S1/Sv

hematopoietic system phenotype

abnormal erythrocyte physiology
- glycerol permeability is decreased relative to wild-type cells

abnormal erythrocyte osmotic lysis
- cells are more resistant to glycerol-induced lysis with a half-time of 14 minutes compared to 2.5 minutes for wild-type cells

decreased erythrocyte osmotic fragility
- cells are more resistant to glycerol-induced lysis with a half-time of 14 minutes compared to 2.5 minutes for wild-type cells

immune system phenotype

decreased susceptibility to parasitic infection
- Normal - however, all mice are dead at day 22 of infection
- mice are more resistant to the early stages of P. berghei with all mice alive at day 12 post-infection whereas 50% of wild-type mice survive at this time point
- male mice exhibit a smaller difference in susceptibility compared to wild-type mice

Genotype: Aqp9^tm1Nlsn/Aqp9^tm1Nlsn
involves: 129S1/Sv * C57BL/6

homeostasis/metabolism phenotype

increased circulating glycerol level
- non-fasting glycerol levels are 530+/-28 compared to 339+/-24 in wild-type mice
- fasting glycerol levels are 493+/-21 compared to 353+/-16 in wild-type mice

increased circulating triglyceride level
- non-fasting levels are 0.83+/-0.02 compared to 0.53+/-0.05 in wild-type mice
- fasting levels are 0.74+/-0.03 compared to 0.58+/-0.02 in wild-type mice

References

Rojek AM; Skowronski MT; Fuchtbauer EM; Fuchtbauer AC; Fenton RA; Agre P; Frokiaer J; Nielsen S. 2007. Defective glycerol metabolism in aquaporin 9 (AQP9) knockout mice. Proc Natl Acad Sci U S A 104(9):3609-14PubMed: 17360690 MGI: J:123742

Additional - Aqp9^tm1Nlsn related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Aqp9
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the Aqp9^- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37111 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Aqp9-

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Aqp9tm1Nlsn

Allele Symbol: Aqp9tm1Nlsn

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Aqp9tm1Nlsn/Aqp9tm1Nlsninvolves: 129S1/Sv

hematopoietic system phenotype

immune system phenotype

Genotype: Aqp9tm1Nlsn/Aqp9tm1Nlsninvolves: 129S1/Sv * C57BL/6

homeostasis/metabolism phenotype

References

Additional - Aqp9tm1Nlsn related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:Aqp9^-

Aqp9^tm1Nlsn

Allele Symbol: Aqp9^tm1Nlsn

Genotype: Aqp9^tm1Nlsn/Aqp9^tm1Nlsn
involves: 129S1/Sv

Genotype: Aqp9^tm1Nlsn/Aqp9^tm1Nlsn
involves: 129S1/Sv * C57BL/6

Additional - Aqp9^tm1Nlsn related