B6.129S1-Aqp9^tm1Nlsn/Mmjax

Stock number: 024764
Product name: Aqp9^-
Research areas: Diabetes and Obesity Research, Metabolism Research

Description

These Aqp9 knockout mice exhibit increased levels of plasma glycerol and triglycerides and may be useful for studying the role of glycerol and glucose metabolism.

Detailed description

Aquaporin 9 (Aqp9 ) encodes an aquaglyceroporin membrane channel known to conduct water, glycerol and other small solutes. Mice homozygous for this null allele exhibit increased levels of plasma glycerol and triglycerides. This knockout may be useful for studying the role of glycerol and glucose metabolism.

Development

The targeting vector contains a PGK neomycin cassette that was used to replace 55 nucleotides in exon 2. The insertion results in correct translation of the first 47 amino acids followed by 100 random amino acids and a stop codon. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and backcrossed for at least 10 generations. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) mice for 1 generation to establish the colony.

Allele

Symbol: Aqp9^tm1Nlsn
Name: targeted mutation 1, Soren Nielsen
MGI accession ID: MGI:3719452
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Aqp9
Marker name: aquaporin 9
Chromosome: 9
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: A neomycin resistance gene replaced 55 nucleotides in exon 2. The insertion was predicted to result in correct translation of the initial 47 amino acids followed by 100 random amino acids and a stop codon. RT-PCR confirmed correct integration of the mutation. An aberrantly spliced transcript was detected, where exon 1 was fused to exon 3, however, the production of protein from this transcript is unlikely.