These Aqp9 knockout mice exhibit increased levels of plasma glycerol and triglycerides and may be useful for studying the role of glycerol and glucose metabolism.
Aleksandra Rojek, Aarhus University
Aquaporin 9 (Aqp9 ) encodes an aquaglyceroporin membrane channel known to conduct water, glycerol and other small solutes. Mice homozygous for this null allele exhibit increased levels of plasma glycerol and triglycerides.
This knockout may be useful for studying the role of glycerol and glucose metabolism.
The targeting vector contains a PGK neomycin cassette that was used to replace 55 nucleotides in exon 2. The insertion results in correct translation of the first 47 amino acids followed by 100 random amino acids and a stop codon. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and backcrossed for at least 10 generations. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) mice for 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Soren Nielsen|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Aqp9, aquaporin 9|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A neomycin resistance gene replaced 55 nucleotides in exon 2. The insertion was predicted to result in correct translation of the initial 47 amino acids followed by 100 random amino acids and a stop codon. RT-PCR confirmed correct integration of the mutation. An aberrantly spliced transcript was detected, where exon 1 was fused to exon 3, however, the production of protein from this transcript is unlikely.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the Aqp9- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37111 in your Materials and Methods section.