C57BL/6N-Tg(Ddx25-EGFP)1Mld/J

Stock number: 024752
Research areas: Developmental Biology Research, Reproductive Biology Research, Research Tools

Description

GRTH 6.4Kb-Tg transgenic mice have Ddx25 promoter/enhancer sequences driving expression of EGFP in somatic Leydig cells and germ cells. These mice may be useful when studying androgen regulation of GRTH.

Detailed description

GRTH 6.4Kb-Tg transgenic mice have the DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences driving expression of an enhanced green fluorescent protein, EGFP. DDX25, also known as gonadotropin-regulated testicular RNA helicase (GRTH), is a testis-specific posttranscriptional regulator of genes that are essential for spermatid elongation and completion of spermiogenesis. Hemizygotes are viable and fertile. GRTH 6.4Kb-Tg mice express EGFP in both somatic Leydig cells and germ cells. When treated with androgen receptor antagonist, flutamide, to obliterate the action of endogenous circulating testosterone, EGFP expression is abolished. Similar to this strain, GRTH 6.4Kb/del-Tg transgenic mice (Stock No. 024753), contain a 5’ deletion - 3.6 kb/-205 bp upstream of the Ddx25 promoter and only express EGFP in germ cells. These mice are less sensitive to flutamide treatment.

Development

The GRTH 6.4Kb-Tg transgene was designed in the laboratory of Dr. Maria L Dufau (Molecular Endocrinology NICHD, NIH) to contain an enhanced green fluorescent protein, EGFP, driven by DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences. The transgene was microinjected into fertilized C57BL/6N oocytes and founder mice were bred to C57BL/6N inbred mice for at least 15 generations to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) to establish the colony.

Allele

Symbol: Tg(Ddx25-EGFP)1Mld
Name: transgene insertion 1, Maria L Dufau
MGI accession ID: MGI:5568392
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Ddx25-EGFP)1Mld
Marker name: transgene insertion 1, Maria L Dufau
Chromosome: UN
Strain of origin: C57BL/6N
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP is expressed in both somatic Leydig cells and germ cells.
Molecular note: The transgene contains an enhanced green fluorescent protein, EGFP, driven by DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences. GFP expression is directed to germ cells and Leydig cells.