GRTH 6.4Kb-Tg transgenic mice have Ddx25 promoter/enhancer sequences driving expression of EGFP in somatic Leydig cells and germ cells. These mice may be useful when studying androgen regulation of GRTH.
Maria Dufau, NIH
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
GRTH 6.4Kb-Tg transgenic mice have the DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences driving expression of an enhanced green fluorescent protein, EGFP. DDX25, also known as gonadotropin-regulated testicular RNA helicase (GRTH), is a testis-specific posttranscriptional regulator of genes that are essential for spermatid elongation and completion of spermiogenesis. Hemizygotes are viable and fertile. GRTH 6.4Kb-Tg mice express EGFP in both somatic Leydig cells and germ cells. When treated with androgen receptor antagonist, flutamide, to obliterate the action of endogenous circulating testosterone, EGFP expression is abolished. Similar to this strain, GRTH 6.4Kb/del-Tg transgenic mice (Stock No. 024753), contain a 5’ deletion - 3.6 kb/-205 bp upstream of the Ddx25 promoter and only express EGFP in germ cells. These mice are less sensitive to flutamide treatment.
The GRTH 6.4Kb-Tg transgene was designed in the laboratory of Dr. Maria L Dufau (Molecular Endocrinology NICHD, NIH) to contain an enhanced green fluorescent protein, EGFP, driven by DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences. The transgene was microinjected into fertilized C57BL/6N oocytes and founder mice were bred to C57BL/6N inbred mice for at least 15 generations to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | EGFP is expressed in both somatic Leydig cells and germ cells. |
Allele Name | transgene insertion 1, Maria L Dufau |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | 6.4Kb-Tg |
Gene Symbol and Name | Tg(Ddx25-EGFP)1Mld, transgene insertion 1, Maria L Dufau |
Gene Synonym(s) | |
Promoter | Ddx25, DEAD (Asp-Glu-Ala-Asp) box polypeptide 25, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed in both somatic Leydig cells and germ cells. |
Strain of Origin | C57BL/6N |
Chromosome | UN |
Molecular Note | The transgene contains an enhanced green fluorescent protein, EGFP, driven by DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences. GFP expression is directed to germ cells and Leydig cells. |
When maintaining a live colony, hemizygous mice may be bred with wildtype (non-carrier) mice from the colony. The donating investigator has not tried to make this strain homozygous.
When using the C57BL/6N-Tg(Ddx25-EGFP)1Mld/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024752 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizyous or Non Carrir for Tg(Ddx25-EGFP)1Mld |
Frozen Mouse Embryo | C57BL/6N-Tg(Ddx25-EGFP)1Mld/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6N-Tg(Ddx25-EGFP)1Mld/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6N-Tg(Ddx25-EGFP)1Mld/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6N-Tg(Ddx25-EGFP)1Mld/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.