| Allele Name | transgene insertion 34, Elaine Fuchs |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | TCF-betagal; Tg(Fos-lacZ)34Efu; TOPGAL; Top-Gal |
| Gene Symbol and Name | Tg(TCF/Lef1-lacZ)34Efu, transgene insertion 34, Elaine Fuchs |
| Gene Synonym(s) | |
| Promoter | Fos, Fos proto-oncogene, AP-1 transcription factor subunit, rat |
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| Expressed Gene | lacZ, beta-galactosidase, E. coli |
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| Site of Expression | lacZ expression occurs during early embryonic development in a subset of pluripotent embryonic basel cells of the epithelium and dermis of developing hair follicles. lacZ expression disappears during formation of hair germ and then reappears at E16.5 in hair follicles until 18 days after birth. |
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| Strain of Origin | Not Specified |
| Chromosome | UN |
| General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgenic mice express beta-galactosidase in the presence of the lymphoid enhancer binding factor 1/transcription factor 3 (LEF1/TCF3) mediated signaling pathway and activated Beta-catenin (CATNB). Transgenic mice display Beta-galactosidase activity during early embryonic development in a subset of pluripotent embryonic basal cells of the epithelium and dermis of developing hair follicles. Beta-galactosidase activity is not detected in the next stage of hair follicle development, formation of hair germs. At E16.5, transgene activity reappears in hair follicles and is detectable until 18 days after birth. |
| Molecular Note | The transgene contains the lacZ gene under the control of a promoter consisting of three consensus lymphoid enhancer binding factor 1/transcription factor 3 (LEF/TCF)-binding motifs upstream of a minimal Fos promoter. This allele is responsive to canonical Wnt/beta-catenin signal transduction. |
| Mutations Made By | Elaine Fuchs, The Rockefeller University |
