These thy1-YFP-H transgenic mice express yellow fluorescent protein at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. This line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons.
Dr. Simon John, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of Stock No. 003709. The primary difference between these two strains is the specific neuron subsets which express YFP. In this strain, a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebellar cortex, but no neurons are labeled in this area. In short, this line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. Fluorescence appears somewhat dim at birth but brightens in adult mice. Availability of multiple spectral variants is useful for double-labeling applications or breeding to other CFP/GFP lines.
This strain is on a genetic background different from that on which the allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kbp fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and crossed with wildtype C57BL/6J mice. This strain was then backcrossed to DBA/2J for at least 22 generations by the donating lab.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Site of Expression | Sensory neurons in dorsal root ganglia, and pyramidal cells in Layer 5 of the cerebral cortex and in the hippocampus express YFP, 10-30% of retinal ganglion cells are exclusively labeled in the retina, mossy fibers are strongly labeled in cerebellar cortex. |
Allele Name | transgene insertion H, Joshua R Sanes |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Tg(Thy1-YFPH)2Jrs; TgN(Thy1-YFP-H)2Jrs; thy1-YFP line H; thy1-YFP-H; thy1-YFP-Htg; YFP-H |
Gene Symbol and Name | Tg(Thy1-YFP)HJrs, transgene insertion H, Joshua R Sanes |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | Sensory neurons in dorsal root ganglia, and pyramidal cells in Layer 5 of the cerebral cortex and in the hippocampus express YFP, 10-30% of retinal ganglion cells are exclusively labeled in the retina, mossy fibers are strongly labeled in cerebellar cortex. |
Strain of Origin | (C57BL/6J x CBA)F1 |
Chromosome | UN |
General Note | Transgenic mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. In these mice a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebellar cortex, but no neurons are labeled in this area. In short, this line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. Fluorescence appears somewhat dim at birth but brightens in adult mice. |
Molecular Note | The transgene contains a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. The regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4, but lacking exon 3 and its flanking introns. Nine lines were generated (12, 16, 21, A, C, D, F, G, and H). |
Mutations Made By | Joshua Sanes, Harvard University |
Hemizygotes are viable and fertile.
When using the thy1-YFP-H mouse strain in a publication, please cite the originating article(s) and include JAX stock #024705 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Thy1-YFP)HJrs |
Frozen Mouse Embryo | D2.Cg-Tg(Thy1-YFP)HJrs/SjJ | $2595.00 |
Frozen Mouse Embryo | D2.Cg-Tg(Thy1-YFP)HJrs/SjJ | $2595.00 |
Frozen Mouse Embryo | D2.Cg-Tg(Thy1-YFP)HJrs/SjJ | $3373.50 |
Frozen Mouse Embryo | D2.Cg-Tg(Thy1-YFP)HJrs/SjJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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