| Allele Name | transgene insertion H, Joshua R Sanes |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | Tg(Thy1-YFPH)2Jrs; TgN(Thy1-YFP-H)2Jrs; thy1-YFP line H; thy1-YFP-H; thy1-YFP-Htg; YFP-H |
| Gene Symbol and Name | Tg(Thy1-YFP)HJrs, transgene insertion H, Joshua R Sanes |
| Gene Synonym(s) | |
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
|---|
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
|---|
| Site of Expression | Sensory neurons in dorsal root ganglia, and pyramidal cells in Layer 5 of the cerebral cortex and in the hippocampus express YFP, 10-30% of retinal ganglion cells are exclusively labeled in the retina, mossy fibers are strongly labeled in cerebellar cortex. |
|---|
| Strain of Origin | (C57BL/6J x CBA)F1 |
| Chromosome | UN |
| General Note | Transgenic mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. In these mice a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebellar cortex, but no neurons are labeled in this area. In short, this line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. Fluorescence appears somewhat dim at birth but brightens in adult mice. |
| Molecular Note | The transgene contains a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. The regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4, but lacking exon 3 and its flanking introns. Nine lines were generated (12, 16, 21, A, C, D, F, G, and H). |
| Mutations Made By | Joshua Sanes, Harvard University |
