P0-3.9GFPCre mice express an EGFP-Cre recombinase fusion protein under the control of the Pax6 P0 enhancer. This strain may be useful for genetic manipulations and visualization during the development of retinal and pancreatic lineages.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
P0-3.9GFPCre mice express a green fluorescent protein (GFP) fused to a Cre recombinase gene under the control of the murine (paired box 6) Pax6 P0 enhancer. The Pax6 P0 enhancer, located 3.9kb upstream of the Pax6 promoter, drives expression in the retinal and pancreatic primordial cells during early embryonic development. Specifically, expression is seen in retinal horizontal cell precursors and pancreatic endocrine and ductal progenitors. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in these cells.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of these B6-congenic mice could vary from that originally described on a STOCK genetic background. We may modify the strain description if necessary as published results become available.
A transgenic construct containing a green fluorescent protein (GFP) fused to a Cre recombinase gene under the control of the murine (paired box 6) Pax6 P0 enhancer was introduced into FVB donor oocytes. Resulting offspring were bred to 129S mice for one generation, and subsequently to C57BL/6 for one generation. Upon arrival at The Jackson Laboratory, mice were backcrossed to C57BL/6J (Stock No. 000664) for at least five generations to establish a congenic colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | The Pax6 promoter drives expression of an EGFP-Cre recombinase fusion protein in the retinal and pancreatic primordial cells during early embryonic development. |
Allele Name | transgene insertion 1, Richard L Maas |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | P0-3.9GFPCre |
Gene Symbol and Name | Tg(Pax6-GFP/cre)1Rilm, transgene insertion 1, Richard L Maas |
Gene Synonym(s) | |
Promoter | Pax6, paired box 6, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | The Pax6 promoter drives expression of an EGFP-Cre recombinase fusion protein in the retinal and pancreatic primordial cells during early embryonic development. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | A transgenic construct containing a sequence encoding a GFP/cre fusion protein under the control of P0 enhancer located 3.9 kb upstream of the murine paired box 6 (Pax6) promoter was introduced into FVB donor oocytes. |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice (noncarriers) from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024688 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non Carrier for Tg(Pax6-GFP/cre)1Rilm |
Frozen Mouse Embryo | B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.