B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J

Stock number: 024688

Description

P0-3.9GFPCre mice express an EGFP-Cre recombinase fusion protein under the control of the Pax6 P0 enhancer. This strain may be useful for genetic manipulations and visualization during the development of retinal and pancreatic lineages.

Detailed description

P0-3.9GFPCre mice express a green fluorescent protein (GFP) fused to a Cre recombinase gene under the control of the murine (paired box 6) Pax6 P0 enhancer. The Pax6 P0 enhancer, located 3.9kb upstream of the Pax6 promoter, drives expression in the retinal and pancreatic primordial cells during early embryonic development. Specifically, expression is seen in retinal horizontal cell precursors and pancreatic endocrine and ductal progenitors. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in these cells.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of these B6-congenic mice could vary from that originally described on a STOCK genetic background. We may modify the strain description if necessary as published results become available.

Development

A transgenic construct containing a green fluorescent protein (GFP) fused to a Cre recombinase gene under the control of the murine (paired box 6) Pax6 P0 enhancer was introduced into FVB donor oocytes. Resulting offspring were bred to 129S mice for one generation, and subsequently to C57BL/6 for one generation. Upon arrival at The Jackson Laboratory, mice were backcrossed to C57BL/6J (Stock No. 000664) for at least five generations to establish a congenic colony.

Allele

Symbol: Tg(Pax6-GFP/cre)1Rilm
Name: transgene insertion 1, Richard L Maas
MGI accession ID: MGI:5538391
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Pax6-GFP/cre)1Rilm
Marker name: transgene insertion 1, Richard L Maas
Chromosome: UN
Strain of origin: FVB
Expressed genes: GFP,Green Fluorescent Protein,; cre,cre recombinase,bacteriophage P1
Site of expression: The Pax6 promoter drives expression of an EGFP-Cre recombinase fusion protein in the retinal and pancreatic primordial cells during early embryonic development.
Molecular note: A transgenic construct containing a sequence encoding a GFP/cre fusion protein under the control of P0 enhancer located 3.9 kb upstream of the murine paired box 6 (Pax6) promoter was introduced into FVB donor oocytes.