Overview

Mycl1^gfp/gfp mice may be useful for studying the role of L-Myc expressing dendritic cells during inflammation and T-cell priming.

Donating Investigator

Dr. Kenneth Murphy, Washington Univ School of Medicine

Genetic overview

Genetic Background	Generation

Mycl^tm1.1Kmm

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Mycl	v-myc avian myelocytomatosis viral oncogene lung carcinoma derived

View Genetics

Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Mycl1^gfp/gfp mutant mice have an enhanced green fluorescent protein sequence replacing the first coding exon of the v-myc myelocytomatosis viral oncogene homolog, lung carcinoma derived (Mycl) gene, abolishing gene expression. Mice that are homozygous for this allele are viable and fertile. GFP fluorescence is visualized in dendritic cells (DCs) in lymphoid and non-lymphoid peripheral tissues. Specifically, Mycl1^gfp was expressed by CD11b⁺ and CD11b^- conventional dendritic cells (cDCs) in the small intestine, and in CD11b^- cDCs in the lung, liver and dermis. Mycl1^gfp was also expressed by CD4^- B220^- cells in small intestinal lamina propria, inside villi and within Peyer's patches. Mycl1^gfp-expressing DCs were found in the T-cell zones of spleen and lymph nodes. Mature DCs express L-Myc in the presence of inflammatory signals such as granulocyte-macrophage colony-stimulating factor (GMSF). Mycl1-deficient mice develop all DC cell types, except CD103⁺ migratory DC numbers in the lung and liver are reduced. Loss of L-Myc in DCs in these mice also causes a decrease in T-cell priming during infection by Listeria monocytogenes and vesicular stomatitis virus.

Development

A targeting vector was designed to replace the first coding exon of the v-myc myelocytomatosis viral oncogene homolog, lung carcinoma derived (Mycl) gene with an enhanced green fluorescent protein sequence and a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived EDJ22 embryonic stem (ES) cells. Correctly targeted ES cells were injected into 129S6/SvEvTac blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. These mice were bred to C57BL/6J mice for at least 7 generations. Subsequently, these mice were bred to B6.C-Tg(CMV-cre)1Cgn/J transgenic mice (Stock No. 006054) to remove the neo cassette. Resulting mice were bred together to remove the cre-expressing transgene. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP fluorescence is visualized in dendritic cells in lymphoid and non-lymphoid peripheral tissues.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

KC W; Satpathy AT; Rapaport AS; Briseno CG; Wu X; Albring JC; Russler-Germain EV; Kretzer NM; Durai V; Persaud SP; Edelson BT; Loschko J; Cella M; Allen PM; Nussenzweig MC; Colonna M; Sleckman BP; Murphy TL; Murphy KM. 2014. L-Myc expression by dendritic cells is required for optimal T-cell priming. Nature 507(7491):243-7PubMed: 24509714 MGI: J:206430

View All References

Genetics

Mycl^tm1.1Kmm

Allele Symbol: Mycl^tm1.1Kmm

Allele Name	targeted mutation 1.1, Kenneth Murphy
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	targeted mutation 1.1, Kenneth Murphy; Mycl^tm1.1Kmm
Gene Symbol and Name	Mycl, v-myc avian myelocytomatosis viral oncogene lung carcinoma derived
Gene Synonym(s)	bHLHe38; Mycl1; MYCL1; lung carcinoma myc related oncogene 1; Lmyc1; Lmyc-1; LMYC; Lmyc-1; Lmyc1; v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian); AW536278; expressed sequence AW536278; L-Myc; L-myc; Mycl1
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP fluorescence is visualized in dendritic cells in lymphoid and non-lymphoid peripheral tissues.
Strain of Origin	129S6/SvEvTac
Chromosome	4
Molecular Note	The targeting vector was designed to replace the first coding exon with a green fluorescent protein (GFP) sequence and a loxP-flanked neomycin resistance (neo) cassette. Mutant mice were bred with Tg(CMV-cre)1Cgn mice (on a C57BL/6 congenic background) to remove the neo selection cassette. qPCR failed to detect mRNA in mutants. GFP expression is detected in dendritic cells in both lymphoid and non-lymphoid peripheral tissues.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers
- Fluorescent Proteins
Immunology, Inflammation and Autoimmunity Research
- Inflammation

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Mycl^tm1.1Kmm/Mycl^tm1.1Kmm
B6.129S6(C)-Mycl

hematopoietic system phenotype

decreased dendritic cell number
- largest reduction is to CD103+ CD11b- conventional dendritic cells in the lung
- total number and relative frequency of dendritic cells is decreased

immune system phenotype

abnormal dendritic cell physiology
- CD8a+ cDC from mice treated with the cytokine, GM-CSF, fail to exhibit improved survival
- decreased proliferation of splenic CD8a+ conventional dendritic cells (cDC) as measured by BrdU incorporation (50% reduction)
- T cell priming by dendritic cells is impaired during infection using either L. monocytogenes expressing soluble ovalbumin (LM-OVA) or VSV-OVA

decreased dendritic cell number
- largest reduction is to CD103+ CD11b- conventional dendritic cells in the lung
- total number and relative frequency of dendritic cells is decreased

decreased susceptibility to bacterial infection induced morbidity/mortality
- mice are resistant to lethal infection by L. monocytogenes

mortality/aging

decreased susceptibility to bacterial infection induced morbidity/mortality
- mice are resistant to lethal infection by L. monocytogenes

References

KC W; Satpathy AT; Rapaport AS; Briseno CG; Wu X; Albring JC; Russler-Germain EV; Kretzer NM; Durai V; Persaud SP; Edelson BT; Loschko J; Cella M; Allen PM; Nussenzweig MC; Colonna M; Sleckman BP; Murphy TL; Murphy KM. 2014. L-Myc expression by dendritic cells is required for optimal T-cell priming. Nature 507(7491):243-7PubMed: 24509714 MGI: J:206430

Additional - Mycl^tm1.1Kmm related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Mycl^tm1.1Kmm
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Wild-type x Heterozygote
- Heterozygote x Wild-type
Citation
When using the B6.129S6(C)-Mycl^tm1.1Kmm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024666 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Mycl<tm1.1Kmm>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6.129S6(C)-Mycl<tm1.1Kmm>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.129S6(C)-Mycl<tm1.1Kmm>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.129S6(C)-Mycl<tm1.1Kmm>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6.129S6(C)-Mycl<tm1.1Kmm>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Mycltm1.1Kmm

Allele Symbol: Mycltm1.1Kmm

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Mycltm1.1Kmm/Mycltm1.1KmmB6.129S6(C)-Mycl

hematopoietic system phenotype

immune system phenotype

mortality/aging

References

Additional - Mycltm1.1Kmm related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Mycl^tm1.1Kmm

Allele Symbol: Mycl^tm1.1Kmm

Genotype: Mycl^tm1.1Kmm/Mycl^tm1.1Kmm
B6.129S6(C)-Mycl

Additional - Mycl^tm1.1Kmm related