B6.129S6(C)-Mycl^tm1.1Kmm/J

Stock number: 024666
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Mycl1^gfp/gfp mice may be useful for studying the role of L-Myc expressing dendritic cells during inflammation and T-cell priming.

Detailed description

Mycl1^gfp/gfp mutant mice have an enhanced green fluorescent protein sequence replacing the first coding exon of the v-myc myelocytomatosis viral oncogene homolog, lung carcinoma derived (Mycl) gene, abolishing gene expression. Mice that are homozygous for this allele are viable and fertile. GFP fluorescence is visualized in dendritic cells (DCs) in lymphoid and non-lymphoid peripheral tissues. Specifically, Mycl1^gfp was expressed by CD11b⁺ and CD11b^- conventional dendritic cells (cDCs) in the small intestine, and in CD11b^- cDCs in the lung, liver and dermis. Mycl1^gfp was also expressed by CD4^- B220^- cells in small intestinal lamina propria, inside villi and within Peyer's patches. Mycl1^gfp-expressing DCs were found in the T-cell zones of spleen and lymph nodes. Mature DCs express L-Myc in the presence of inflammatory signals such as granulocyte-macrophage colony-stimulating factor (GMSF). Mycl1-deficient mice develop all DC cell types, except CD103⁺ migratory DC numbers in the lung and liver are reduced. Loss of L-Myc in DCs in these mice also causes a decrease in T-cell priming during infection by Listeria monocytogenes and vesicular stomatitis virus.

Development

A targeting vector was designed to replace the first coding exon of the v-myc myelocytomatosis viral oncogene homolog, lung carcinoma derived (Mycl) gene with an enhanced green fluorescent protein sequence and a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived EDJ22 embryonic stem (ES) cells. Correctly targeted ES cells were injected into 129S6/SvEvTac blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. These mice were bred to C57BL/6J mice for at least 7 generations. Subsequently, these mice were bred to B6.C-Tg(CMV-cre)1Cgn/J transgenic mice (Stock No. 006054) to remove the neo cassette. Resulting mice were bred together to remove the cre-expressing transgene. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Mycl^tm1.1Kmm
Name: targeted mutation 1.1, Kenneth Murphy
MGI accession ID: MGI:5550274
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Mycl
Marker name: v-myc avian myelocytomatosis viral oncogene lung carcinoma derived
Chromosome: 4
Strain of origin: 129S6/SvEvTac
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP fluorescence is visualized in dendritic cells in lymphoid and non-lymphoid peripheral tissues.
Molecular note: The targeting vector was designed to replace the first coding exon with a green fluorescent protein (GFP) sequence and a loxP-flanked neomycin resistance (neo) cassette. Mutant mice were bred with Tg(CMV-cre)1Cgn mice (on a C57BL/6 congenic background) to remove the neo selection cassette. qPCR failed to detect mRNA in mutants. GFP expression is detected in dendritic cells in both lymphoid and non-lymphoid peripheral tissues.