B6;129-Gdf7^tm1Kng/J

Stock number: 024649
Research areas: Reproductive Biology Research

Description

Gdf7 homozygous knockout animals have reduced viability and males are sterile due to a significant reduction in the size of the seminal vesicles.

Detailed description

Gdf7 (growth differentiation factor 7) is a member of the bone morphogenetic protein (BMP) family that is an essential signaling protein required for the normal growth, branching, and functional differentiation of seminal vesicle epithelium.

These targeted mutant mice carry a knockout of the mouse Gdf7 gene. Homozygous males are sterile due to a significant reduction in the size of the seminal vesicles. The remainder of the male reproductive tract appears normal. Viability of homozygotes is reduced and many die around 8 weeks of age due to hydrocephalus.

Development

The entire mature coding domain was replaced by a neomycin resistance cassette via homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Resultant chimeric mice were backcrossed to C57BL/6 for an unknown number of generations by the donating laboratory.

Allele

Symbol: Gdf7^tm1Kng
Name: targeted mutation 1, David M Kingsley
MGI accession ID: MGI:2387817
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Gdf7-
Marker symbol: Gdf7
Marker name: growth differentiation factor 7
Marker synonyms: BMP12
Chromosome: 12
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: The entire mature coding region was replaced with a neomycin resistance cassette via homologous recombination. Homozygous mutant animals were identified by PCR genotype analysis.