Gdf7 homozygous knockout animals have reduced viability and males are sterile due to a significant reduction in the size of the seminal vesicles.
David Kingsley, Stanford University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Gdf7 | growth differentiation factor 7 |
Gdf7 (growth differentiation factor 7) is a member of the bone morphogenetic protein (BMP) family that is an essential signaling protein required for the normal growth, branching, and functional differentiation of seminal vesicle epithelium.
These targeted mutant mice carry a knockout of the mouse Gdf7 gene. Homozygous males are sterile due to a significant reduction in the size of the seminal vesicles. The remainder of the male reproductive tract appears normal. Viability of homozygotes is reduced and many die around 8 weeks of age due to hydrocephalus.
The entire mature coding domain was replaced by a neomycin resistance cassette via homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant chimeric mice were backcrossed to C57BL/6 for an unknown number of generations by the donating laboratory.
Allele Name | targeted mutation 1, David M Kingsley |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Gdf7- |
Gene Symbol and Name | Gdf7, growth differentiation factor 7 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 12 |
Molecular Note | The entire mature coding region was replaced with a neomycin resistance cassette via homologous recombination. Homozygous mutant animals were identified by PCR genotype analysis. |
Heterozygotes are viable and fertile. Viability of homozygotes is reduced and many die around 8 weeks of age due to hydrocephalus. Surviving homozygous males are sterile.
When using the B6;129-Gdf7tm1Kng/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024649 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Gdf7<tm1Kng> |
Frozen Mouse Embryo | B6;129-Gdf7<tm1Kng>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gdf7<tm1Kng>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gdf7<tm1Kng>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Gdf7<tm1Kng>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.