Gdf7 homozygous knockout animals have reduced viability and males are sterile due to a significant reduction in the size of the seminal vesicles.
David Kingsley, Stanford University School of Medicine
Gdf7 (growth differentiation factor 7) is a member of the bone morphogenetic protein (BMP) family that is an essential signaling protein required for the normal growth, branching, and functional differentiation of seminal vesicle epithelium.
These targeted mutant mice carry a knockout of the mouse Gdf7 gene. Homozygous males are sterile due to a significant reduction in the size of the seminal vesicles. The remainder of the male reproductive tract appears normal. Viability of homozygotes is reduced and many die around 8 weeks of age due to hydrocephalus.
The entire mature coding domain was replaced by a neomycin resistance cassette via homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant chimeric mice were backcrossed to C57BL/6 for an unknown number of generations by the donating laboratory.
|Allele Name||targeted mutation 1, David M Kingsley|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Gdf7, growth differentiation factor 7|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The entire mature coding region was replaced with a neomycin resistance cassette via homologous recombination. Homozygous mutant animals were identified by PCR genotype analysis.|
Heterozygotes are viable and fertile. Viability of homozygotes is reduced and many die around 8 weeks of age due to hydrocephalus. Surviving homozygous males are sterile.
When using the B6;129-Gdf7tm1Kng/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024649 in your Materials and Methods section.