A neo cassette replaces exons 2-5 of the lipocalin 2 (Lcn2) gene, abolishing gene expression. LCN2 is an iron-trafficking protein involved in apoptosis and the regulation of the innate immune systems response to bacterial growth and infection. Toll-like receptors on innate immune cells stimulate the production and secretion of LCN2 upon bacterial infection. LCN2 limits bacterial growth by iron sequestration, which is required by bacteria in order to replicate. Homozygous LCN2-deficient mice are viable and fertile and should be housed in a specific pathogen free (SPF) facility. Escherichia coli challenge causes an increase in bacterial burden in the liver and spleen along with physical signs of sepsis such as lethargy, hunched posture, and ruffled fur. E Coli infection caused no difference in leukocyte numbers, neutrophil infiltration, or tissue iron distribution. Also, after induction of accelerated nephrotoxic serum nephritis (NTS), LCN2 KO mice exhibit more glomerular damage with increased proteinuria and interstitial leukocyte accumulation than wildtype mice.
A targeting vector was designed to replace exons 2-5 of the lipocalin 2 (Lcn2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females. These mice were backcrossed for at least 10 generations to C57BL/6J mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Alan Aderem|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Lcn2, lipocalin 2|
|Gene Synonym(s)||24p3; AW212229; MSFI; NGAL; Sip24; expressed sequence AW212229; p25|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A neo replaced coding exons 2-5. Absence of transcript was demonstrated by Northern blot analysis of mutant peritoneal macrophages. Immunoblot of mutant cell lysates confirmed the lack of protein.|
When maintaining a live colony, homozygous mice may be bred together. Mice should be housed in a specific pathogen free (SPF) facility.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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