B6.Cg-Myo10^m1J/GrsrJ

Stock number: 024583

Description

This spontaneous mutation in myosin X causes syndactyly in the front feet, white belly spots, irregular tail pigment, and ocular abnormalities such as persistent hyaloid vasculature, corneal opacity, and iris coloboma

Detailed description

Mice homozygous for the Myo10^m1J spontaneous mutation have white spots on the belly and, more rarely, on the back of an otherwise black C57BL/6J coat. They are slightly smaller than normal, often have irregular regions of diminished pigment on the tail, and also have fused digits on either or both of the front feet. This syndactyly has not been found in the digits of the hind feet. Ocular abnormalities caused by this mutation include corneal opacity, iris coloboma, lens spot, and persistent hyaloid vasculature.

Development

The Myo10 mutation arose spontaneously in the strain B6.129S2-Cd4^tm1Mak/J at The Jackson Laboratory in early 2012. The Cd4^tm1Mak mutation was bred out of this mutant subline by two backcrosses to C57BL/6J. Sperm was cryopreserved from homozygous males.

Allele

Symbol: Myo10^m1J
Name: mutation 1 Jackson
MGI accession ID: MGI:5578506
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Myo10
Marker name: myosin X
Chromosome: 15
Strain of origin: B6.129S2-Cd4^tm1Mak/J
Molecular note: This is a spontaneous eight nucleotide (CGACGAGT) deletion at chromosome 15 position 25,781,267-274 bp (GRCm38). The deletion disrupts exon 25 and produces a frame shift expected to truncate the full-length and all three headless transcripts following aa 948. Immunoblot of whole brain lysates from 1-month-old mutant mice revealed combined deletion of the full-length and headless forms, confirming complete loss of encoded protein.