Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.
Rosa26ERG mice conditionally expresses the human ERG transcription factor after Cre-mediated excision of a loxP-flanked STOP sequence. It is useful in studying ETS-mediated prostate oncogenesis.
Charles L. Sawyers, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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N6pN1
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Starting at:
$255.00 Domestic price for female |
$2,854.50 Domestic price Cryo Recovery |
Translocation of ETS transcription factors ERG (avian erythroblastosis virus E-26 (v-ets) oncogene related), ETV1, ETV4, EV5, and FLI1 occur in half of all prostate cancers. TMPRSS2 (transmembrane protease, serine 2)-ERG translocation is the most common molecular alteration.
These Rosa26ERG mice conditionally express the TMPRSS2-ERG fusion transcription factor as well as a nuclear-localized EGFP from the Gt(ROSA26)Sor gene after cre-mediated excision of a loxP-flanked Stop sequence. When crossed into probasin (Pbsn)-cre, ERG is specifically and strongly expressed in the prostate, causing a low penetrance of prostate hyperplasia. When bred with Pbsn-cre and Pten floxed mice (to knock out PTEN expression in the prostate), ERG expression causes accelerated, highly penetrant invasive prostate cancer in the progeny. Triple mutant mice show invasive adenocarcinoma characterized by small irregular glandular structures composed of malignant cells with large, pleiomorphic nuclei and pale cytoplasm developed adjacent to prostatic intraepithelial neoplasia by 8 weeks of age. By 6 months, approximately 80% of these mice contain regions of adenocarcinoma with enlarged, hardened prostates.
EGFP expression can be detected directly or through immunohistochemistry.
A splice acceptor (SA), PGK-neomycin and 4x-polyA transcriptional Stop between reverse orientation loxP sites, TMPRSS2 (non-coding human exon 1)-ERG (exon 4) cDNA, IRES-EGFP with 3 nuclear localization sequences (nls), and a polyA tail were inserted into intron 1 of the non-coding Gt(ROSA)26Sor gene. The targeting vector was introduced to 129-derived embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for 6 generations by the donating laboratory.
Expressed Gene | TMPRSS2, transmembrane serine protease 2, human |
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Expressed Gene | ERG, ETS transcription factor ERG, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP and the TMPRSS2-ERG fusion transcription factor will be expressed in cells where promoters driving Cre recombinase are expressed. |
Allele Name | targeted mutation 1, Charles L Sawyers |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Rosa26ERG |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | TMPRSS2, transmembrane serine protease 2, human |
Expressed Gene | ERG, ETS transcription factor ERG, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP and the TMPRSS2-ERG fusion transcription factor will be expressed in cells where promoters driving Cre recombinase are expressed. |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | The targeting construct introduced into the locus a splice acceptor sequence, floxed neomycin resistance STOP (4x polyadenylation sequence) cassette, fusion of human TMPRSS2 (exon 1) and ERG (exon 4), IRES, nuclear localized EGFP and polyadenylation sequence. |
Heterozygotes and homozygotes are viable and fertile.
When using the Rosa26ERG/ERG mouse strain in a publication, please cite the originating article(s) and include JAX stock #024512 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(TMPRSS2/ERG)Key> |
Frozen Mouse Embryo | B6.129-Gt(ROSA)26Sor<tm1(TMPRSS2/ERG)Key>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Gt(ROSA)26Sor<tm1(TMPRSS2/ERG)Key>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Gt(ROSA)26Sor<tm1(TMPRSS2/ERG)Key>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Gt(ROSA)26Sor<tm1(TMPRSS2/ERG)Key>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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