B6;CBA-Tg(Tbx21-cre)1Dlc/J

Stock number: 024507
Product name: Tbet-cre
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice express Cre recombinase from the mouse Tbx21 (T-box 21; also called Tbet) promoter in mitral cells of the main and accessory olfactory bulbs.

Detailed description

These transgenic mice express Cre recombinase from the mouse Tbx21 (T-box 21; also called Tbet) promoter in mitral cells of the main and accessory olfactory bulbs. Surveys of the rest of the brain show that Cre-induced recombination is specific to the olfactory bulbs and occurs by E14.5 in the developing mitral cell layer. Cre-mediated recombination has also been demonstrated in the thymus. Homozygotes are viable and fertile, and are phenotypically normal.

Mitral and tufted cells demonstrate lacZ staining in progeny derived from crosses with a floxed STOP lacZ reporter strain (e.g. Stock No. 003474).

Crosses with a floxed Stop hChR2(H134R)-tdTomato fluorescent reporter strain (see Stock No. 012567) enable direct optogenetic activation of mitral and tufted cells in the olfactory bulb.

Development

Cre cDNA sequences were introduced to the start ATG of the Tbx21 gene on mouse BAC RP23-237M14. The ~200 kb linearized BAC, free of its vector, was introduced to C57BL/6 x CBA oocytes. A founder line determined to have strong cre expression, as determined by anti-cre antibody staining and olfactory bulb recombination activity, was minimally backcrossed to C57BL/6J by the donating laboratory.

Allele

Symbol: Tg(Tbx21-cre)1Dlc
Name: transgene insertion 1, Catherine Dulac
MGI accession ID: MGI:5528860
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Tbx21-cre)1Dlc
Marker name: transgene insertion 1, Catherine Dulac
Chromosome: UN
Strain of origin: C57BL/6 x CBA
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: The T-box 21 (Tbx21) promoter drives the expression of cre recombinase in mitral cells of the main and accessory olfactory bulbs.
Molecular note: Cre cDNA was inserted at the start ATG of the Tbx21 gene in a BAC (RP23-237M14). The recombined BAC was linearized and injected into B6/CBA oocytes. 14 animals were generated and one was determined to be positive for the Tbx21-cre transgene. The single founder line was analyzed by immunohistochemistry for cre protein. Strong expression was detected and reporter crosses displayed recombination in the mitral and external tufted layers of the main and accessory olfactory bulbs, with no other brain tissues showing recombination. Activity was also observed in the thymus, a known site of Tbx21 expression.