These mutant mice carry Stat4 and Ifngr1 knock out alleles, the Tg(TcraBDC2.5,TcrbBDC2.5)1Doi and Thy1a, and are defective in generation of Th1 cells. This strain has applications in studies of Type 1 diabetes and for adoptive transfer studies.
Linda M Bradley, Sanford Burnham Medical Research Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Stat4 | signal transducer and activator of transcription 4 |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ifngr1 | interferon gamma receptor 1 |
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | Thy1 | thymus cell antigen 1, theta |
These mice are homozygous for the Stat4tm1Gru, Thy1a Ifngr1tm1Agt targeted mutation alleles, homozygous for the Thy1a spontaneous mutation allele and hemizygous for the Tg(TcraBDC2.5,TcrbBDC2.5)1Doi transgene. Unable to respond to IL12 and IFNgamma, the mutant mice are defective in generation of Th1 cells.
Homozygote NOD.129S2-Stat4tm1Gru/JbsJ (Stock No. 004671) mice, homozygote NOD.NON-Thy1a/1LtJ (Stock No. 004483) mice, mice on the NOD background homozygous for the Ifngr1tm1Agt allele and mice on the NOD background hemizygous for the Tg(TcraBDC2.5,TcrbBDC2.5)1Doi transgene were bred together to generate this mutant strain.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression | |
Site of Expression | The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. |
Allele Name | targeted mutation 1, Michael J Grusby |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Stat4- |
Gene Symbol and Name | Stat4, signal transducer and activator of transcription 4 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | The exon encoding amino acids 263 through 314 was disrupted by the insertion of a neomycin selection cassette. The encoded protein was undetectable in the thymi and lymph nodes of homozygous mutant mice by immunoblot analysis. |
Mutations Made By | Dr. Michael Grusby, Harvard Medical School |
Allele Name | targeted mutation 1, Michel Aguet |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | G129; IFn type II receptor; IFN-gamma R-; IFN-gamma R0; IFN-gammaR-; IFNgammaR KO; IFN-gammaR KO; IFN-gamma-R KO; IFN-gammaR-; IFN-gammaR0; IFN-gammaR1-; IFN-gammaRalpha_; Ifngr-; IFNgR KO; Ifngr1- |
Gene Symbol and Name | Ifngr1, interferon gamma receptor 1 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 10 |
Molecular Note | Insertion of a neomycin cassette into exon V, which encodes an extracellular membrane-proximal portion of the receptor. |
Mutations Made By | Dr. Michel Aguet, EPFL, Ecole polytechnique fédérale de Lausanne |
Allele Name | transgene insertion 1, Christophe Benoist |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | BDC2.5; BDC-2.5/N TCR Tg; Tg(TcraBDC2.5)2Doi; Tg(TcrbBDC2.5)2Doi |
Gene Symbol and Name | Tg(TcraBDC2.5,TcrbBDC2.5)1Doi, transgene insertion 1, Christophe Benoist |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | This transgene results from the coinjection of two constructs derived from diabetogenic T-cell clone BDC2.5. The Tcra construct contains a rearranged Tcra sequence (V alpha J alpha) that begins 20 bp 5' of the ATG site and ends 30 bp 3' of the splice donor sequence of J alpha 17. The 6.5 Tcrb construct contains rearranged Tcrb sequence (V beta D beta J beta) flanked by 2 kb of 5' regulatory sequences and 3.5 kb of 3' DNA containing the unrearranged J beta 1.3 to J beta 1.6 sequences, and precedes a 12 kb sequence containing the C beta 2 region. |
Allele Name | a variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a |
Gene Symbol and Name | Thy1, thymus cell antigen 1, theta |
Gene Synonym(s) | |
Site of Expression | The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. |
Strain of Origin | Not Applicable |
Chromosome | 9 |
General Note | The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900). |
Molecular Note | The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains. |
When maintaining a live colony, these mice can be bred as homozygotes for the Stat4tm1Gru, Thy1a, and Ifngr1tm1Agt alleles and hemizygous for the Tg(TcraBDC2.5,TcrbBDC2.5)1Doi transgene.
When using the (NOD.STAT4 KO x NOD BDC Thy1.1) x (NOD.IFNgR KO) mouse strain in a publication, please cite the originating article(s) and include JAX stock #024476 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Stat4<tm1Gru>, Homozygous for Thy1<a>, Homozygous for Ifngr1<tm1Agt>,Hemizygous or non carrier for Tg(TcraBDC2.5,TcrbBDC2.5)1Doi |
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