Overview

Also Known As:(NOD.STAT4 KO x NOD BDC Thy1.1) x (NOD.IFNgR KO)

These mutant mice carry Stat4 and Ifngr1 knock out alleles, the Tg(TcraBDC2.5,TcrbBDC2.5)1Doi and Thy1^a, and are defective in generation of Th1 cells. This strain has applications in studies of Type 1 diabetes and for adoptive transfer studies.

Donating Investigator

Linda M Bradley, Sanford Burnham Medical Research Institute

Genetic overview

Genetic Background	Generation

Stat4^tm1Gru

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Stat4	signal transducer and activator of transcription 4

Ifngr1^tm1Agt

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Ifngr1	interferon gamma receptor 1

Tg(TcraBDC2.5,TcrbBDC2.5)1Doi

Allele Type
Transgenic (Inserted expressed sequence)

Thy1^a

Allele Type	Gene Symbol	Gene Name
Not Applicable	Thy1	thymus cell antigen 1, theta

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools
Diabetes and Obesity Research
Cancer Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

These mice are homozygous for the Stat4^tm1Gru, Thy1^a Ifngr1^tm1Agt targeted mutation alleles, homozygous for the Thy1^a spontaneous mutation allele and hemizygous for the Tg(TcraBDC2.5,TcrbBDC2.5)1Doi transgene. Unable to respond to IL12 and IFNgamma, the mutant mice are defective in generation of Th1 cells.

Development

Homozygote NOD.129S2-Stat4^tm1Gru/JbsJ (Stock No. 004671) mice, homozygote NOD.NON-Thy1^a/1LtJ (Stock No. 004483) mice, mice on the NOD background homozygous for the Ifngr1^tm1Agt allele and mice on the NOD background hemizygous for the Tg(TcraBDC2.5,TcrbBDC2.5)1Doi transgene were bred together to generate this mutant strain.

Expression Data

Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Site of Expression
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.

Control Suggestions

Approximate Controls

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Selected References

Christianson SW; Shultz LD; Leiter EH. 1993. Adoptive transfer of diabetes into immunodeficient NOD-scid/scid mice. Relative contributions of CD4+ and CD8+ T-cells from diabetic versus prediabetic NOD.NON-Thy-1a donors. Diabetes 42(1):44-55PubMed: 8093606 MGI: J:34990
Huang S; Hendriks W; Althage A; Hemmi S; Bluethmann H; Kamijo R; Vilcek J; Zinkernagel RM; Aguet M. 1993. Immune response in mice that lack the interferon-gamma receptor [see comments] Science 259(5102):1742-5PubMed: 8456301 MGI: J:63815
Kaplan MH; Sun YL; Hoey T; Grusby MJ. 1996. Impaired IL-12 responses and enhanced development of Th2 cells in Stat4-deficient mice. Nature 382(6587):174-7PubMed: 8700209 MGI: J:34059
Katz JD; Wang B; Haskins K; Benoist C; Mathis D. 1993. Following a diabetogenic T cell from genesis through pathogenesis. Cell 74(6):1089-100PubMed: 8402882 MGI: J:77007

View All References

Genetics

Stat4^tm1Gru

Allele Symbol: Stat4^tm1Gru

Allele Name	targeted mutation 1, Michael J Grusby
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Stat4-
Gene Symbol and Name	Stat4, signal transducer and activator of transcription 4
Gene Synonym(s)
Strain of Origin	129S2/SvPas
Chromosome	1
Molecular Note	The exon encoding amino acids 263 through 314 was disrupted by the insertion of a neomycin selection cassette. The encoded protein was undetectable in the thymi and lymph nodes of homozygous mutant mice by immunoblot analysis.
Mutations Made By	Dr. Michael Grusby, Harvard Medical School

Ifngr1^tm1Agt

Allele Symbol: Ifngr1^tm1Agt

Allele Name	targeted mutation 1, Michel Aguet
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	G129; IFn type II receptor; IFN-gamma R-; IFN-gamma R⁰; IFN-gammaR-; IFNgammaR KO; IFN-gammaR KO; IFN-gamma-R KO; IFN-gammaR^-; IFN-gammaR⁰; IFN-gammaR1^-; IFN-gammaRalpha^_; Ifngr-; IFNgR KO; Ifngr1^-
Gene Symbol and Name	Ifngr1, interferon gamma receptor 1
Gene Synonym(s)
Strain of Origin	129S7/SvEvBrd-Hprt⁺
Chromosome	10
Molecular Note	Insertion of a neomycin cassette into exon V, which encodes an extracellular membrane-proximal portion of the receptor.
Mutations Made By	Dr. Michel Aguet, EPFL, Ecole polytechnique fédérale de Lausanne

Tg(TcraBDC2.5,TcrbBDC2.5)1Doi

Allele Symbol: Tg(TcraBDC2.5,TcrbBDC2.5)1Doi

Allele Name	transgene insertion 1, Christophe Benoist
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	BDC2.5; BDC-2.5/N TCR Tg; Tg(TcraBDC2.5)2Doi; Tg(TcrbBDC2.5)2Doi
Gene Symbol and Name	Tg(TcraBDC2.5,TcrbBDC2.5)1Doi, transgene insertion 1, Christophe Benoist
Gene Synonym(s)
Promoter	Tcrb, T cell receptor beta chain, mouse, laboratory
Promoter	Tcra, T cell receptor alpha chain, mouse, laboratory
Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcra, T cell receptor alpha chain, mouse, laboratory
Strain of Origin	(C57BL/6 x SJL)F2
Chromosome	UN
Molecular Note	This transgene results from the coinjection of two constructs derived from diabetogenic T-cell clone BDC2.5. The Tcra construct contains a rearranged Tcra sequence (V alpha J alpha) that begins 20 bp 5' of the ATG site and ends 30 bp 3' of the splice donor sequence of J alpha 17. The 6.5 Tcrb construct contains rearranged Tcrb sequence (V beta D beta J beta) flanked by 2 kb of 5' regulatory sequences and 3.5 kb of 3' DNA containing the unrearranged J beta 1.3 to J beta 1.6 sequences, and precedes a 12 kb sequence containing the C beta 2 region.

Thy1^a

Allele Symbol: Thy1^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	thetaAKR; theta-AKR; Thy1.1; Thy-1.1; Thy1a
Gene Symbol and Name	Thy1, thymus cell antigen 1, theta
Gene Synonym(s)
Site of Expression	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Strain of Origin	Not Applicable
Chromosome	9
General Note	The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1^b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).
Molecular Note	The allele Thy1^a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Thy1^a related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker
  - Tissue/Cell Markers

Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
  - Type 1 Diabetes
Diabetes and Obesity Research
- Type 1 Diabetes (IDDM)

Genotype: Ifngr1^tm1Agt related

Cancer Research
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Genotype: Stat4^tm1Gru related

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
- Intracellular Signaling Molecules

Mammalian Phenotype Terms by Genotype

References

Christianson SW; Shultz LD; Leiter EH. 1993. Adoptive transfer of diabetes into immunodeficient NOD-scid/scid mice. Relative contributions of CD4+ and CD8+ T-cells from diabetic versus prediabetic NOD.NON-Thy-1a donors. Diabetes 42(1):44-55PubMed: 8093606 MGI: J:34990
Huang S; Hendriks W; Althage A; Hemmi S; Bluethmann H; Kamijo R; Vilcek J; Zinkernagel RM; Aguet M. 1993. Immune response in mice that lack the interferon-gamma receptor [see comments] Science 259(5102):1742-5PubMed: 8456301 MGI: J:63815
Kaplan MH; Sun YL; Hoey T; Grusby MJ. 1996. Impaired IL-12 responses and enhanced development of Th2 cells in Stat4-deficient mice. Nature 382(6587):174-7PubMed: 8700209 MGI: J:34059
Katz JD; Wang B; Haskins K; Benoist C; Mathis D. 1993. Following a diabetogenic T cell from genesis through pathogenesis. Cell 74(6):1089-100PubMed: 8402882 MGI: J:77007

Additional - Ifngr1^tm1Agt related

Additional - Stat4^tm1Gru related

Additional - Thy1^a related

Additional - Tg(TcraBDC2.5,TcrbBDC2.5)1Doi related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- End Point Analysis:Thy1 End Point
- End Point Analysis:Thy1 End Point
- Standard PCR:Tg(TcraBDC2.5)1Doi, Tg(TcrbBDC2.5)2Doi
- Standard PCR:Ifngr1-Alternate 1
- Standard PCR:Stat4
- Standard PCR:Tg(TcraBDC2.5)1Doi, Tg(TcrbBDC2.5)2Doi
- Probe:Tg(TcraBDC2.5,TcrbBDC2.5)1Doi Probe
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes for the Stat4^tm1Gru, Thy1^a, and Ifngr1^tm1Agt alleles and hemizygous for the Tg(TcraBDC2.5,TcrbBDC2.5)1Doi transgene.
- Additional Breeding and Husbandry Support
Citation
When using the (NOD.STAT4 KO x NOD BDC Thy1.1) x (NOD.IFNgR KO) mouse strain in a publication, please cite the originating article(s) and include JAX stock #024476 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Homozygous for Stat4<tm1Gru>, Homozygous for Thy1<a>, Homozygous for Ifngr1<tm1Agt>,Hemizygous or non carrier for Tg(TcraBDC2.5,TcrbBDC2.5)1Doi

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:(NOD.STAT4 KO x NOD BDC Thy1.1) x (NOD.IFNgR KO)

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Stat4tm1Gru

Allele Symbol: Stat4tm1Gru

Ifngr1tm1Agt

Allele Symbol: Ifngr1tm1Agt

Tg(TcraBDC2.5,TcrbBDC2.5)1Doi

Allele Symbol: Tg(TcraBDC2.5,TcrbBDC2.5)1Doi

Thy1a

Allele Symbol: Thy1a

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Thy1a related

Genotype: Ifngr1tm1Agt related

Genotype: Stat4tm1Gru related

Mammalian Phenotype Terms by Genotype

References

Additional - Ifngr1tm1Agt related

Additional - Stat4tm1Gru related

Additional - Thy1a related

Additional - Tg(TcraBDC2.5,TcrbBDC2.5)1Doi related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Stat4^tm1Gru

Allele Symbol: Stat4^tm1Gru

Ifngr1^tm1Agt

Allele Symbol: Ifngr1^tm1Agt

Thy1^a

Allele Symbol: Thy1^a

Genotype: Thy1^a related

Genotype: Ifngr1^tm1Agt related

Genotype: Stat4^tm1Gru related

Additional - Ifngr1^tm1Agt related

Additional - Stat4^tm1Gru related

Additional - Thy1^a related