This targeted mutant allele of Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1); also called GluR1) incorporates a deletion of exon 11.
Richard L Huganir, Johns Hopkins University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Gria1 | glutamate receptor, ionotropic, AMPA1 (alpha 1) |
The Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1); also called GluR1) gene encodes an AMPA receptor subunit that has been found to regulate synaptic plasticity.
This targeted mutant allele incorporates a deletion of exon 11 which encodes transmembrane domains 1 and 2. A presumably truncated form of the protein is weakly expressed.
Exon 11 (encoding transmembrane domains 1 and 2) was replaced with a neomycin resistance cassette through homologous recombination in C57BL/6-derived R1 embryonic stem (ES) cells. This strain was maintained on a C57BL/6 genetic background by the donating lab.
Allele Name | targeted mutation 3, Richard Huganir |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GluR1- |
Gene Symbol and Name | Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1) |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 11 |
Molecular Note | A neomycin resistance gene replaced the exon encodeing transmembrane domains one and two. The presumably truncated protein was expressed weakly in mutants. |
Heterozygotes are viable and fertile, but homozygotes reportedly do not breed well.
When using the C57BL/6-Gria1tm3Rlh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024422 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wildtype for Gria1<tm3Rlh> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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