This targeted mutant allele of Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1); also called GluR1) incorporates a deletion of exon 11.
Richard L Huganir, Johns Hopkins University School of Medicine
The Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1); also called GluR1) gene encodes an AMPA receptor subunit that has been found to regulate synaptic plasticity.
This targeted mutant allele incorporates a deletion of exon 11 which encodes transmembrane domains 1 and 2. A presumably truncated form of the protein is weakly expressed.
Exon 11 (encoding transmembrane domains 1 and 2) was replaced with a neomycin resistance cassette through homologous recombination in C57BL/6-derived R1 embryonic stem (ES) cells. This strain was maintained on a C57BL/6 genetic background by the donating lab.
|Allele Name||targeted mutation 3, Richard Huganir|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1)|
|Strain of Origin||C57BL/6|
|Molecular Note||A neomycin resistance gene replaced the exon encodeing transmembrane domains one and two. The presumably truncated protein was expressed weakly in mutants.|
Heterozygotes are viable and fertile, but homozygotes reportedly do not breed well.
When using the C57BL/6-Gria1tm3Rlh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024422 in your Materials and Methods section.