C57BL/6-Gria1^tm3Rlh/J

Stock number: 024422
Research areas: Neurobiology Research

Description

This targeted mutant allele of Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1); also called GluR1) incorporates a deletion of exon 11.

Detailed description

The Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1); also called GluR1) gene encodes an AMPA receptor subunit that has been found to regulate synaptic plasticity.

This targeted mutant allele incorporates a deletion of exon 11 which encodes transmembrane domains 1 and 2. A presumably truncated form of the protein is weakly expressed.

Development

Exon 11 (encoding transmembrane domains 1 and 2) was replaced with a neomycin resistance cassette through homologous recombination in C57BL/6-derived R1 embryonic stem (ES) cells. This strain was maintained on a C57BL/6 genetic background by the donating lab.

Allele

Symbol: Gria1^tm3Rlh
Name: targeted mutation 3, Richard Huganir
MGI accession ID: MGI:3605221
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gria1
Marker name: glutamate receptor, ionotropic, AMPA1 (alpha 1)
Chromosome: 11
Strain of origin: C57BL/6
Molecular note: A neomycin resistance gene replaced the exon encodeing transmembrane domains one and two. The presumably truncated protein was expressed weakly in mutants.