These mice carry S831D and S845D amino acid substitutions in the mouse Gria1 gene that are phosphomimetic (mimic a phosphorylated protein). Phosphorylation at these amino acids is sufficient to lower the threshold for LTP induction, increasing the probability of synaptic plasticity.
Richard L Huganir, Johns Hopkins University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted | Gria1 | glutamate receptor, ionotropic, AMPA1 (alpha 1) |
Phosphorylation of Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1); also known as GluA1) has been proposed to regulate receptor trafficking and synaptic transmission and plasticity.
These mice carry S831D and S845D amino acid substitutions in the mouse Gria1 gene that are phosphomimetic (mimic a phosphorylated protein). Mice show normal total and surface expression and subcellular localization of GLUA1 as well as intact basal synaptic transmission. Unlike wildtype animals, mutant mice show long-term potentiation (LTP) induced with a weak 10-Hz stimulation. Evidence indicates that phosphorylation of GLUA1 at S831 and S845 is sufficient to lower the threshold for LTP induction, increasing the probability of synaptic plasticity.
S831D (TCC->GAT) and S845D (AGC->GAC) serine to aspartate mutations were introduced to GluA1 (Gria1) cDNA using QuikChange site-directed mutagenesis (Stratagene). The mutations were introduced to 129S6/SvEvTac-derived MC1 embryonic stem (ES) cells by homologous recombination. This strain was backcrossed to C57BL/6 for 10 generations by the donating laboratory.
Allele Name | targeted mutation 7, Richard L Huganir |
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Allele Type | Targeted |
Allele Synonym(s) | GluA1 S831D/S845D; GluA1DD |
Gene Symbol and Name | Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1) |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 11 |
Molecular Note | S831D (TCC to GAT) and S845D (AGC to GAC) serine to aspartate mutations were introduced to GluA1 (Gria1) cDNA using QuikChange site-directed mutagenesis (Stratagene). |
Homozygotes and heterozygotes are viable and fertile.
When using the B6.129S6-Gria1tm7Rlh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024418 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Gria1<tm7Rlh> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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