By three weeks of age mice homozygous for the spontaneous mutation shaky all over (sao) have a shaking gait and whole body tremor, which continues throughout their lifespan. Histology of two males at thirty-four weeks of age doubly homozygous for Il10tm1Cgn and sao on a predominantly NOD background revealed severe Purkinje cell degeneration, consistent with the neurological phenotype. Mothers on this double homozygous NOD background generally develop rectal prolapse after one to three litters, but this is not found in sao homozygotes on this C57BL/6J congenic background, which lacks the Il10 targeted allele.
The shaky all over mutation arose spontaneously in the strain NOD.129P2(Cg)-Il10tm1Cgn/Dvs at The Jackson Laboratory. This mutation was backcrossed onto the C57BL/6J background for 7 backcross generations and then sibling intercrossed and sperm was cryopreserved from homozygous males.
|Allele Name||shaky all over|
|Allele Type||Spontaneous (Not Specified)|
|Gene Symbol and Name||sao, shaky all over|
|Strain of Origin||NOD.129P2(Cg)-Il10tm1Cgn/Dvs|
|Molecular Note||This heritable phenotypic marker arose spontaneously at The Jackson Laboratory.|