These transgenic mice express green fluorescent calcium indicator GCaMP6f in subsets of excitatory neurons in the brain and possibly other cell types and tissues. The transgene contains a mouse Thy1 promoter, GCaMP6f, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. The GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics (<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=23868258">PMID 23868258</a>). The mice can be used for imaging-based monitoring of neuronal activity in individual neurons in the brain and elsewhere. Stable expression levels in the cortex (motor cortex, somatosensory cortex, and visual cortex of neocortex layers 2/3 and 5) and hippocampus (CA1, CA3, and dentate gyrus) are sufficient for in vivo imaging. L2/3 functional imaging experiments require 50-100mW average laser power (940 nm) as compared to alternative 10-25 mW in GCaMP viral transduction protocols. These mice were made by the Genetically-Encoded Neuronal Indicator and Effector (<a href="http://www.janelia.org/team-project/genie">GENIE</a>) Project at the HHMI's Janelia Farm Research Campus.

Please see the <a href="http://Jackson.jax.org/rs/444-BUH-304/images/24275_24276_24339_Neuroscience_2013_Kim_poster.pdf">Neuroscience, 2013 poster</a> for more information. A selection of <a href="http://Jackson.jax.org/rs/444-BUH-304/images/24275_24276_24339_Neuroscience_2013_Kim_poster.pdf">whole brain widefield images</a> and an
<a href="http://Jackson.jax.org/rs/444-BUH-304/images/GCamP6_expression.pdf">expression summary table</a> are also available.

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These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector (<a href="http://www.janelia.org/team-project/genie">GENIE</a>) Project, express the green fluorescent calcium indicator, GCaMP6f, in subsets of excitatory neurons in the brain and possibly other cell types and tissues. The GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics.

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