C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J

Stock number: 024339
Product name: GP5.11
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project, express the green fluorescent calcium indicator, GCaMP6f, in subsets of excitatory neurons in the brain and possibly other cell types and tissues. The GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics.

Detailed description

These transgenic mice express green fluorescent calcium indicator GCaMP6f in subsets of excitatory neurons in the brain and possibly other cell types and tissues. The transgene contains a mouse Thy1 promoter, GCaMP6f, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. The GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics (PMID 23868258). The mice can be used for imaging-based monitoring of neuronal activity in individual neurons in the brain and elsewhere. Stable expression levels in the cortex (motor cortex, somatosensory cortex, and visual cortex of neocortex layers 2/3 and 5) and hippocampus (CA1, CA3, and dentate gyrus) are sufficient for in vivo imaging. L2/3 functional imaging experiments require 50-100mW average laser power (940 nm) as compared to alternative 10-25 mW in GCaMP viral transduction protocols. These mice were made by the Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project at the HHMI's Janelia Farm Research Campus.

Please see the Neuroscience, 2013 poster for more information. A selection of whole brain widefield images and an expression summary table are also available.

Development

The mouse Thy1 promoter drives expression of this transgene that includes a mutagenized green fluorescent calcium indicator (GCaMP6f), woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. GCaMP consists of a circularly permutated green fluorescent protein (cpGFP), the calcium-binding protein calmodulin (CaM), and CaM-interacting M13 peptide. This strain was created and maintained on a C57BL/6J genetic background by the donating laboratory.

Allele

Symbol: Tg(Thy1-GCaMP6f)GP5.11Dkim
Name: transgene insertion GP5.11, Douglas Kim
MGI accession ID: MGI:5523980
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Thy1-GCaMP6f)GP5.11Dkim
Marker name: transgene insertion GP5.11, Douglas Kim
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: GCaMP,Genetically encoded calcium indicator,
Site of expression: Green fluorescent calcium indicator, GCaMP6f, is expressed in subsets of excitatory neurons in the brain and possibly other cell types and tissues.
Molecular note: The mouse promoter drives neuron-specific expression of this transgene that includes a mutagenized green fluorescent calcium indicator (GCaMP6f), woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. GCaMP consists of a circularly permutated green fluorescent protein (cpGFP), the calcium-binding protein calmodulin (CaM), and CaM-interacting M13 peptide. This GCaMP6f variant of GCaMP incorporates A317E, T381R, S383T, and R392G mutations in CaM. Lines GP5.5 and GP5.11 were generated.