These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project, express the green fluorescent calcium indicator, GCaMP6f, in subsets of excitatory neurons in the brain and possibly other cell types and tissues. The GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics.
Douglas Kim, Janelia Farm Research Campus, HHMI
Genetic Background | Generation |
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N3F6
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Allele Type |
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Transgenic (Reporter) |
Starting at:
$278.00 Domestic price for female |
356.51 Domestic price for breeder pair |
These transgenic mice express green fluorescent calcium indicator GCaMP6f in subsets of excitatory neurons in the brain and possibly other cell types and tissues. The transgene contains a mouse Thy1 promoter, GCaMP6f, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. The GCaMP6f indicator is an ultrasensitive detector of single neuronal action potentials and has fast response kinetics (PMID 23868258). The mice can be used for imaging-based monitoring of neuronal activity in individual neurons in the brain and elsewhere. Stable expression levels in the cortex (motor cortex, somatosensory cortex, and visual cortex of neocortex layers 2/3 and 5) and hippocampus (CA1, CA3, and dentate gyrus) are sufficient for in vivo imaging. L2/3 functional imaging experiments require 50-100mW average laser power (940 nm) as compared to alternative 10-25 mW in GCaMP viral transduction protocols. These mice were made by the Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project at the HHMI's Janelia Farm Research Campus.
Please see the Neuroscience, 2013 poster for more information. A selection of whole brain widefield images and an
expression summary table are also available.
The mouse Thy1 promoter drives expression of this transgene that includes a mutagenized green fluorescent calcium indicator (GCaMP6f), woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. GCaMP consists of a circularly permutated green fluorescent protein (cpGFP), the calcium-binding protein calmodulin (CaM), and CaM-interacting M13 peptide. This strain was created and maintained on a C57BL/6J genetic background by the donating laboratory.
Expressed Gene | GCaMP, Genetically encoded calcium indicator, |
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Site of Expression | Green fluorescent calcium indicator, GCaMP6f, is expressed in subsets of excitatory neurons in the brain and possibly other cell types and tissues. |
Allele Name | transgene insertion GP5.11, Douglas Kim |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | GP5.11 |
Gene Symbol and Name | Tg(Thy1-GCaMP6f)GP5.11Dkim, transgene insertion GP5.11, Douglas Kim |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | GCaMP, Genetically encoded calcium indicator, |
Site of Expression | Green fluorescent calcium indicator, GCaMP6f, is expressed in subsets of excitatory neurons in the brain and possibly other cell types and tissues. |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | The mouse promoter drives neuron-specific expression of this transgene that includes a mutagenized green fluorescent calcium indicator (GCaMP6f), woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation sequence. GCaMP consists of a circularly permutated green fluorescent protein (cpGFP), the calcium-binding protein calmodulin(CaM), and CaM-interacting M13 peptide. This GCaMP6f variant of GCaMP incorporates A317E, T381R, S383T, and R392G mutations in CaM. Lines GP5.5 and GP5.11 were generated. |
Hemizygotes are viable and fertile.
When using the GP5.11 mouse strain in a publication, please cite the originating article(s) and include JAX stock #024339 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Thy1-GCaMP6f)GP5.11Dkim |
Frozen Mouse Embryo | C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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