These floxed mutant mice possess loxP sites flanking exon 5 of the Abl1 gene. This strain may be useful for generating conditional mutations in applications related to regulation of cytoskeletal reorganization in response to extracellular stimuli.
Anthony J Koleske, Yale University
The Abl1 protooncogene encodes for a nonreceptor tyrosine kinase which is involved processes such as signal transduction in cytoskeleton reorganization, stress response, cell adhesion and differentiation, apoptosis, and phagocytosis. These mice possess loxP sites on either side of exon 5 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 5 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in neuronal lineage cells, this mutant mouse strain may be useful in studies of dendrite branching.
A loxP site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 5 of the targeted gene, and another loxP site was inserted upstream of exon 5. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone cre-mediated recombination and no longer retained the cassette but did retain the loxP-flanked exon 5 were injected into recipient blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were backcrossed to C57BL/6 for 8 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Anthony J Koleske|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||ablflox; c-Ablflox|
|Gene Symbol and Name||Abl1, c-abl oncogene 1, non-receptor tyrosine kinase|
|Strain of Origin||129S4/SvJae|
|Molecular Note||LoxP sites were inserted to flank exon 5.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the c-Ablflox , ablflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #024286 in your Materials and Methods section.