These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Cd28 gene. This strain may be useful for generating conditional mutations in applications related to T cell activation, proliferation, and survival.
Laurence A. Turka, Massachusetts General Hospital
Costimulatory CD28 is necessary for activation and proliferation of T cells. These mice possess loxP sites on either side of exon 2 and 3 of the Cd28 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 and 3 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in FOXP3+ Treg cells (see Stock No. 016959 for example), this mutant mouse strain may be useful in studies of regulatory T cells suppression of autoimmunity.
A FRT-site site flanked PGK-NEO casette and single loxP site was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into C57BL/6 derived PB6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Heterozygotes were crossed to generate homozygotes.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Laurence A Turka|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Cd28, CD28 antigen|
|Strain of Origin||C57BL/6|
|General Note||ES cell line = PB6 (C57BL/6)|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 3.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C57BL/6-Cd28tm1Ltu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024282 in your Materials and Methods section.