The transcription initiation codon and the secretory signal recognition peptide sequence, of the fibrinogen-like protein 1 (Fgl1) gene have been replaced with a loxP-flanked neomycin resistance (neo) cassette, abolishing gene expression. FGL1 is expressed in the liver and is involved in hepatocyte proliferation and repair. It has been found overexpressed in hepatocellular carcinomas and following acute liver injury. Homozygotes are viable and fertile. Fgl1-/- mice are 37% heavier than wild type mice at three weeks of age. They are also longer and contain more adipose tissue. These mice have decreased plasma cholesterol, free fatty acid levels, and fasting hyperglycemia with enhanced gluconeogenesis. Adipose tissue morphology is abnormal in Fgl1-/- mice. Brown adipose tissue contains larger lipid droplets and has reduced number of nuclei, while white adipocytes are larger and fewer in number than in wildtype mice.
A targeting vector was designed to replace exons 2-3, including the transcription initiation codon, of the fibrinogen-like protein 1 (Fgl1) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into (129S6/SvEvTac x C57BL/6J)F1-derived PTL1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6 females. Fgl1-/- mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Chinweike Ukomadu|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Fgl1, fibrinogen-like protein 1|
|Strain of Origin||(129S6/SvEvTac x C57BL/6J)F1|
|Molecular Note||A floxed neomycin resistance cassette replaced exons 2 and 3. Western blot analysis confirmed the absence of protein in the liver.|
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129S6-Fgl1tm1Cuko/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024004 in your Materials and Methods section.