Overview

These targeted mutant mice carry a knockout allele of the Matn1 gene (matrilin 1, cartilage matrix protein). Cartilage shows abnormal type II collagen fibrillogenesis and fibril organization.

Donating Investigator

Qian Chen, Rhode Island Hospital, Brown University

Genetic overview

Genetic Background	Generation

Matn1^tm1Goe

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Matn1	matrilin 1, cartilage matrix protein

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Research Applications

Internal/Organ Research
Cell Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Matrilin-1 (cartilage matrix protein; Matn1) is a homotrimeric protein that forms collagen-dependent and collagen-independent fibrils in the extracelluar matrix of cartilage.

These targeted mutant mice carry a knockout allele of the Matn1 gene. Ultrastructural studies of the cartilage of long bone growth plates from homozygotes reveals an abnormal type II collagen fibrillogenesis and fibril organization in the matrix of the zone of maturation. In contrast to the meshwork-like organization of collagen fibrils in wildtype cartilage, the fibrils of matrilin null animals are unidirectional. Fibrils in the mutant cartilage also become significantly larger in diameter than those of controls. The cartilage matrix is stiffer in knockout mice, and the knee joint is prone to injury-induced osteoarthritis.

Reduced matrilin content in the pericellular matrix surrounding chondrocytes decreases their proliferative/gene expression responses to mechanical stimulation. Chondrocytes from the knockout mice cultured under mechanical pressure show only a 0.5-fold increase in mRNA levels of collagen type X, in comparison to the more than 2.5-fold increase seen in wildtype chondrocytes.

Mice heterozygous or homozygous for the targeted allele grow normally and display no major phenotypic differences from the wild-type littermates during skeletal development. Homozygous null mice are fully viable with normal fecundity.

Development

A 1.8 kbp PGK-neo fragment replacing 0.8 kbp of genomic sequence encoding exon 4 and a portion of exon 5 was introduced to 129S4/SvJae-derived J1 embryonic stem (ES) cells by homologous recombination. Targeted ES cells were microinjected into C57BL/6 blastocysts. The resulting chimeric male mice were bred to C57BL/6 females. This strain was maintained on a mixed C57BL/6-129 genetic background prior to sending to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. To generate the living colony, an aliquot of the frozen sperm was used to fertilize oocytes from B6129SF1/J female mice (Stock No. 101043). The mutant mice were then bred together, to wildtype mice from the colony, or to B6129SF1/J mice.

Control Suggestions

Wild-type from the colony

Approximate Controls

101043 B6129SF1/J

Additional Information

Considerations for Choosing Controls

Selected References

Huang X; Birk DE; Goetinck PF. 1999. Mice lacking matrilin-1 (cartilage matrix protein) have alterations in type II collagen fibrillogenesis and fibril organization. Dev Dyn 216(4-5):434-41PubMed: 10633862 MGI: J:58843

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Genetics

Matn1^tm1Goe

Allele Symbol: Matn1^tm1Goe

Allele Name	targeted mutation 1, Paul F Goetinck
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	mat-1^tm1Goe; Matn1-
Gene Symbol and Name	Matn1, matrilin 1, cartilage matrix protein
Gene Synonym(s)
Strain of Origin	129S4/SvJae
Chromosome	4
Molecular Note	A neomycin cassette replaced exon 4 and part of exon 5. In situ hybridization and immunohistochemical analysis in distal growth plates of E15 homozygous embryos demonstrated an absence of transcript and protein.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Internal/Organ Research
- Skeleton
Cell Biology Research
- Signal Transduction

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Matn1^tm1Goe/Matn1^tm1Goe
involves: 129S4/SvJae * C57BL/6

skeleton phenotype

abnormal long bone epiphyseal plate morphology
- exhibit abnormal type II collagen fibrillogenesis and fibril organization in the matrix of the zone of maturation as early as E15 and particularly at P1
- fibrils are bundled in a single direction and show an increase in diameter
- Normal - however, do not show disorganization of the chondrocytes of the growth plate, deposition of matrix molecules such as aggrecan or type X collagen, or abnormal skeletal formation

References

Huang X; Birk DE; Goetinck PF. 1999. Mice lacking matrilin-1 (cartilage matrix protein) have alterations in type II collagen fibrillogenesis and fibril organization. Dev Dyn 216(4-5):434-41PubMed: 10633862 MGI: J:58843

Additional - Matn1^tm1Goe related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Matn1alternate3
- Standard PCR:Generic Neo
- Probe:Generic Neo
- Genotyping resources and troubleshooting
Breeding Considerations

Homozygotes and heterozygotes are viable and fertile.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129S-Matn1^tm1Goe/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024003 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Matn1<tm1Goe>

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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General Terms and Conditions

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Matn1tm1Goe

Allele Symbol: Matn1tm1Goe

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Matn1tm1Goe/Matn1tm1Goeinvolves: 129S4/SvJae * C57BL/6

skeleton phenotype

References

Additional - Matn1tm1Goe related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Matn1^tm1Goe

Allele Symbol: Matn1^tm1Goe

Genotype: Matn1^tm1Goe/Matn1^tm1Goe
involves: 129S4/SvJae * C57BL/6

Additional - Matn1^tm1Goe related