B6.Cg-Tg(Sirpa-tTA)AUmri/J

Stock number: 023970
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice express the tetracycline-controlled transactivator (tTA) protein under the control of the mouse signal-regulatory protein alpha (Sirpa) gene. These DG-A mice provide a tool that allows the inducible expression of genes in neurons of the dentate gyrus, and may be useful for studying memory circuits in vivo.

Detailed description

These DG-A transgenic mice express the tetracycline-controlled transactivator (tTA) protein under the control of the mouse signal-regulatory protein alpha (Sirpa) promoter. Homozygous mice are viable and fertile. SIRPA is a receptor-type transmembrane glycoprotein expressed in the dentate granule cells of the hippocampus. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), the target gene is expressed in neurons of the dentate gyrus, which can be inhibited with administration of the tetracycline analog, doxycycline (dox).

When bred to a beta-galactosidase reporter strain (tetO-lacZ), the resulting double mutant mice exhibit a beta-galactosidase activity in almost all (88%) of mature dentate granule cells (DGCs) as compared to only 37% in DG-S mice (Stock No. 023971).

Development

A mouse bacterial artificial chromosome (BAC), clone 394B7, containing the signal-regulatory protein alpha (Sirpa) gene, was modified by insertion of the tetracycline transactivator gene (tTA) sequence, such that the Sirpa promoter drives expression of the tTA. The BAC was linearized and microinjected into the pronucleus of B6 x SJL fertilized eggs. Founder line A was subsequently established. These mice were then bred to C57BL/6J mice for at least 6 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Sirpa-tTA)AUmri
Name: transgene insertion A, Hisashi Umemori
MGI accession ID: MGI:5516343
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Sirpa-tTA)AUmri
Marker name: transgene insertion A, Hisashi Umemori
Chromosome: UN
Strain of origin: C57BL/6 x SJL
Expressed genes: tTA,tetracycline-controlled transactivator,E. coli
Site of expression: The tetracycline-controlled transactivator (tTA) protein is expressed in the dentate granule cells of the hippocampus under the control of the mouse signal-regulatory protein alpha (Sirpa) promoter.
Molecular note: A mouse bacterial artificial chromosome (BAC), clone 394B7, containing the signal-regulatory protein alpha (Sirpa) gene, was modified by insertion of the tetracycline transactivator gene (tTA) sequence, such that the Sirpa promoter drives expression of the tTA. Lines A and S were generated.