These DG-A transgenic mice express the tetracycline-controlled transactivator (tTA) protein under the control of the mouse signal-regulatory protein alpha (Sirpa) promoter. Homozygous mice are viable and fertile. SIRPA is a receptor-type transmembrane glycoprotein expressed in the dentate granule cells of the hippocampus. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), the target gene is expressed in neurons of the dentate gyrus, which can be inhibited with administration of the tetracycline analog, doxycycline (dox). 

 When bred to a beta-galactosidase reporter strain (tetO-lacZ), the resulting double mutant mice exhibit a beta-galactosidase activity in almost all (88%) of mature dentate granule cells (DGCs) as compared to only 37% in DG-S mice (Stock No. <a href="https://www.jax.org/strain/023971">023971</a>).

These transgenic mice express the tetracycline-controlled transactivator (tTA) protein under the control of the mouse signal-regulatory protein alpha (Sirpa) gene. These DG-A mice provide a tool that allows the inducible expression of genes in neurons of the dentate gyrus, and may be useful for studying memory circuits in vivo.

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