Exon 2 of both the mouse Lin28a and Lin28b genes are flanked by loxP sites in this targeted mutant strain. Cre excision of each floxed region results in a knockout which may be useful in studies of growth and metabolism.
George Q. Daley, Children's Hospital Boston
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Lin28a | lin-28 homolog A (C. elegans) |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Lin28b | lin-28 homolog B (C. elegans) |
Human LIN28A (lin-28 homolog A (C. elegans)) and LIN28B (lin-28 homolog A (C. elegans)) genes have been implicated in growth and glucose metabolism.
In this targeted mutation strain, the exon 2 sequences in both Lin28a and Lin28b have been flanked by loxP sites. Cre excision of the floxed segments creates knockout alleles. Mice homozygous for both floxed alleles are viable and fertile.
Mice homozygous for a knockout of both genes (produced by crosses with germline cre mice, e.g. Stock No. 006954) show developmental delays and embryonic lethality.
Exon 2 of each gene was flanked by loxP sites and an FRT-flanked neomycin cassette was introduced to intron 2 through homologous recombination in (C57BL/6 x 129S4/SvJae)F1-derived v6.5 embryonic stem (ES) cells. Chimeric animals were bred with BALB/c animals then then the line was backcrossed to C57BL/6 for 6 generations (see SNP note below). The neomycin cassettes were excised via a Flp recombinase.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the 27 markers throughout the genome were segregating suggested an incomplete backcross. Also, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic.
Allele Name | targeted mutation 2.1, George Q Daley |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Lin28a, lin-28 homolog A (C. elegans) |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 4 |
General Note | The targeting vector used to generate this allele was co-injected with the targeting vector used to generate Lin28btm2.1Gqda. |
Molecular Note | A loxP site was inserted upstream of exon 2. An FRT flanked neo cassette with a 5' loxP site was inserted downstream of exon 2 via homologous recombination. FLP-mediated recombination removed the neomycin cassette. |
Allele Name | targeted mutation 2.1, George Q Daley |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Lin28b, lin-28 homolog B (C. elegans) |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 10 |
General Note | The targeting vector used to generate this allele was co-injected with the targeting vector used to generate Lin28atm2.1Gqda. |
Molecular Note | A loxP site was inserted upstream of exon 2. An FRT flanked neo cassette with a 5' loxP site was inserted downstream of exon 2 via homologous recombination. FLP-mediated recombination removed the neomycin cassette. |
Mice homozygous for both floxed alleles are viable and fertile.
When using the STOCK Lin28atm2.1Gqda Lin28btm2.1Gqda/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023915 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Lin28a<tm2.1Gqda> Lin28b<tm2.1Gqda> |
Frozen Mouse Embryo | STOCK Lin28a<tm2.1Gqda> Lin28b<tm2.1Gqda>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Lin28a<tm2.1Gqda> Lin28b<tm2.1Gqda>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Lin28a<tm2.1Gqda> Lin28b<tm2.1Gqda>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Lin28a<tm2.1Gqda> Lin28b<tm2.1Gqda>/J Frozen Embryo | $3373.50 |
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