STOCK Setd5^tm1.1Mgn/Mmjax

Stock number: 023898
Research areas: Research Tools

Description

The GFP reporter inserted downstream of the Setd5 promoter eliminates Setd5 transcription (creating a null allele) and provides a reporter for Setd5 expression. SETD5 (SET domain-containing protein 5) is involved in epigenetic repression of gene expression.

Detailed description

Heterozygous mice harboring the Setd5.GFP reporter are viable and fertile. Mice homozygous for the mutation are not viable. Insertion of the GFP reporter downstream of the Setd5 promoter eliminates Setd5 transcription (creating a null allele) and provides a reporter for Setd5 gene expression. Setd5 is located upstream of Rosa26 and is transcribed in the opposite orientation. SETD5 (SET domain-containing protein 5) is involved in epigenetic repression of gene expression.

Development

Using the ROSA26^LCA or Loxed Cassette Acceptor allele (Gt(ROSA)26Sor^tm1Mgn), which contains a loxP-flanked (lox71, lox2272) selection cassette in the ROSA26 promoter, a donor cassette is inserted into the acceptor allele via recombinase mediated cassette exchange (RMCE). The donor cassette or signaling sentinel construct includes an FRT-flanked pgk-hygromycin and a GFP-SV40 polyA reporter sequence.  The GFP reporter inserted into exon1 of the Setd5 gene, which is located upstream of the Rosa26 exon 1 and is transcribed in the opposite orientation. The construct was electroporated into 129S6/SvEvTac-derived TL1 ES cells. Chimeric mice were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J transgenic mice to delete the pgk-hygromycin cassette. The donating investigator reported that the mice were backcrossed to CD-1 mice for 4 generations prior to sending to MMRRC at JAX. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.