This Rara knockout strain is useful in studies of the role of retinoic acid receptors during development.
Henry Sucov, Medical University of South Carolina
The Rara (retinoic acid receptor, alpha) gene encodes for a nuclear ligand dependent transcription factor that is the most abundant and widespread retinoic acid receptor. These mice carry a targeted mutation for the Rara gene in which a NEO cassette replaced exon 2, which is specific to the alpha 1 isoform.
Mice that are homozygous for the targeted mutation are viable, fertile and exhibit no overtly abnormal phenotype. No gene product (mRNA) is detected by Northern blot and RT-PCR analysis on skin, muscle and liver from homozygous animals.
A targeting vector containing a PGK-NEO cassette was used to disrupt exon 2. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then crossed to several other mutant lines with genetic backgrounds including, but not limited to: C57BL/6, ICR (CD-1), and 129Sv.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish this colony of mice carrying only the Raratm1Rev allele.
|Allele Name||targeted mutation 1, Ronald M Evans|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Rara1 null; RARalpha1 -|
|Gene Symbol and Name||Rara, retinoic acid receptor, alpha|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A DNA fragment containing a portion of the coding sequence downstream of the sixth codon was replaced with a neomycin selection cassette. Northern blot and RT-PCR analysis on skin, muscle and liver samples of homozygous mice demonstrated that no detectable transcript is produced from this allele.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the RARalpha1 - mouse strain in a publication, please cite the originating article(s) and include JAX stock #023845 in your Materials and Methods section.