This strain is currently unavailable due to replenishing of cryopreserved stocks.
These SNCA-OVX mice express a transgene containing the human SNCA gene and develop age-dependent motor incoordination and loss of nigrostriatal dopamine neurons. They are suitable for use in applications related to the study of Parkinson disease.
Richard Wade-Martins, University of Oxford
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Snca | synuclein, alpha |
Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These mutant mice carry a transgene containing human SNCA gene and a knock out allele of the mouse Snca gene. Human α-synuclein protein levels in the striatum of mutant mice is 1.9-fold higher than levels of endogenous mouse α-synuclein protein. Transgene expression is detected in the striatum (caudate putamen and nucleus accumbens). Mice that are homozygous for the targeted mutation and the transgene are viable and fertile. Male mice have an increased dry stool weight. SNCA-OVX mice, aged 18 months, exhibit age-related impairment of motor coordination, and loss of nigrostriatal dopamine neurons. Diminishment of motor coordination and firing rate of dopamine neurons of the substantia nigra pars compacta is age-dependent. Dopamine transmission in the dorsal striatum is reduced as early as 3-4 months of age. The transgene was detected at a single site of integration near the centromere of chromosome 4 by fluorescence in situ hybridization analysis.
A BAC construct carrying the complete 111-kb human wildtype SNCA locus and endogenous promoter sequences was microinjected into the pronucleus of CBA X C57BL/6 F1 fertilized eggs. Founder line 37 was subsequently crossed to mice carrying the Sncatm1Rosl allele (see, for example, Stock No. 003692) on a congenic C57BL/6 background for 9 generations. The transgene was detected at a single site of integration near the centromere of chromosome 4 by fluorescence in situ hybridization analysis. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | SNCA, synuclein alpha, human |
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Site of Expression | Transgene expression is detected in the striatum (caudate putamen and nucleus accumbens). |
Allele Name | targeted mutation 1, Arnon Rosenthal |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | alpha-Syn-; alpha-Synko |
Gene Symbol and Name | Snca, synuclein, alpha |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 6 |
Molecular Note | A PGK-neo cassette was used to delete exons 1 and 2, encoding amino acids 1 through 41 as well as a 5' untranslated region. RT-PCR analysis of extracts from the brains of homozygous mutant mice, using probes for both the deleted 5' region as well the untargeted 3' region, showed an absence of transcript. Western blot analysis, immunohistochemical analysis, and in situ hybridization confirmed a lack of encoded protein in homozygous mutant mice. |
Allele Name | transgene insertion OVX37, Richard Wade-Martins |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | SNCA-OVX |
Gene Symbol and Name | Tg(SNCA)OVX37Rwm, transgene insertion OVX37, Richard Wade-Martins |
Gene Synonym(s) | |
Promoter | SNCA, synuclein alpha, human |
Expressed Gene | SNCA, synuclein alpha, human |
Site of Expression | Transgene expression is detected in the striatum (caudate putamen and nucleus accumbens). |
Strain of Origin | (CBA x C57BL/6)F1 |
Chromosome | 4 |
Molecular Note | This transgene consists of a BAC carrying the complete 111-kb wild-type human SNCA locus. This line overexpresses the human protein at nearly twice the endogenous level of the mouse protein. Fluorescence in situ hybridization analysis identified a single site of integration near the centromere of chromosome 4. |
When maintaining a live colony, these mice can be bred as homozygotes for the targeted mutation and the transgene.
When using the SNCA-OVX mouse strain in a publication, please cite the originating article(s) and include JAX stock #023837 in your Materials and Methods section.
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