These floxed mutant mice possess loxP sites flanking exons 2 through 5, and part of exon 6, of the Gabrd gene. This strain may be useful for generating conditional mutations in applications related to the regulation of nervous system electrophysiology and GABAergic inhibition.
Jamie Maguire, Tufts University School of Medicine
The Gabrd (gamma-aminobutyric acid (GABA) A receptor, subunit delta) gene encodes for the delta subunit of the GABAA receptor which is a member of the cys-loop family of ligand-gated ion channels. These mice possess loxP sites flanking exons 2 through 5 and a portion of exon 6 of the targeted gene. Exons 2 through 5 and a portion of exon 6 encode 204 amino acids N-terminal of the extracellular domain. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 5 and a portion of exon 6 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in GAD65-positive interneurons (see Stock No. 010802 for example), this mutant mouse strain may be useful in studies of nervous system electrophysiology and tonic inhibition in interneurons.
A targeting vector containing a loxP site and a FRT site flanked PGK-NEO cassette was utilized in the construction of this mutant. This targeting vector was inserted into exon 6 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into unspecified 129Sv derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
The resulting chimeric animals were crossed to mice expressing FLPe recombinase (on the C57BL/6 genetic background ) to remove the FRT flanked selection cassette. Mice that retained the loxP site flanked region of exon 2 through 5 and a portion of exon 6, were then bred to C57BL/6 mice to remove the FLPe recombinase allele. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Jamie Maguire|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Gabrd, gamma-aminobutyric acid (GABA) A receptor, subunit delta|
|Strain of Origin||129|
|Molecular Note||A targeting vector containing a FRT site flanked PGK-NEO cassette and a loxP site was inserted into exon 6 of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the neomycin resistance cassette and left exons 2 through 5 and a portion of exon 6 floxed.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the floxed Gabrd mouse strain in a publication, please cite the originating article(s) and include JAX stock #023836 in your Materials and Methods section.