Mgl2DTR knockin mice are useful when studying the requirement for CD301b+ dermal dendritic cells in the generation of Th2 cell-mediated immunity.
Akiko Iwasaki, Yale School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Mgl2 | macrophage galactose N-acetyl-galactosamine specific lectin 2 |
Mgl2DTR mutant mice contain a cDNA encoding human diptheria toxin receptor (DTR; formal symbol HBEGF)-EGFP fusion protein in exon 2 of the macrophage galactose N-acetyl-galactosamine specific lectin 2 (Mgl2) gene, abolishing gene expression. MGL2, also known as CD301b, is a transmembrane protein expressed in dermal dendritic cells (DDCs). Following immunization with antigen, CD301b+ DDCs are involved in the differentiation of naive CD4+ T cells into T helper (Th)2 cells. Th2 cells play an important role in cell-mediated immunity against extracellular parasites, bacteria, allergens, and toxins by producing cytokines and regulating B cell class-switching. Homozygous Mgl2DTR mice are viable and fertile. GFP fluorescence is evident in CD301b+ DDCs. Injection of diptheria toxin (DT) specifically depletes CD301b+ DDCs resulting in decreased accumulation and expression of CD69 by polyclonal CD4+ T cells in the skin-draining lymph node. These mice also exhibit impaired Th2 cell development upon infection with Nippostrongylus brasiliensis. Mice receiving multiple injections of DT are viable, and CD301b+ DDCs depletion lasts 6 days after a single DT injection.
The Mgl2DTR targeting vector was designed to insert a human diphtheria toxin receptor (DTR)-enhanced green fluorescent protein (EGFP) cassette, followed by a loxP-flanked neomycin (neo) resistance cassette, into exon 2 of the macrophage galactose N-acetyl-galactosamine specific lectin 2 (Mgl2) gene. This construct was injected into C57BL/6N-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6N blastocysts. Resulting chimeric males were bred with B6.FVB-Tg(EIIa-cre) mice to remove the neo cassette and offspring were crossed to remove the cre-expressing transgene. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.
Expressed Gene | DTR/EGFP, Simian Diphtheria Toxin Receptor; Enhanced Green Fluorescent Protein, |
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Site of Expression | GFP fluorescence is observed in CD301b+ dermal dendritic cells. |
Allele Name | targeted mutation 1.1, Akiko Iwasaki |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | CD301bGFP-DTR; Mgl2DTR-GFP; Mgl2DTR |
Gene Symbol and Name | Mgl2, macrophage galactose N-acetyl-galactosamine specific lectin 2 |
Gene Synonym(s) | |
Expressed Gene | DTR/EGFP, Simian Diphtheria Toxin Receptor; Enhanced Green Fluorescent Protein, |
Site of Expression | GFP fluorescence is observed in CD301b+ dermal dendritic cells. |
Strain of Origin | C57BL/6 |
Chromosome | 11 |
Molecular Note | The targeting vector was designed to insert a human diphtheria toxin receptor (DTR)-enhanced green fluorescent protein (EGFP) fusion cassette, followed by a loxP-flanked neomycin (neo) resistance cassette, into exon 2. Cre-mediated recombination removed the neomycin resistance cassette. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6(FVB)-Mgl2tm1.1(HBEGF/EGFP)Aiwsk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023822 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Mgl2<tm1.1(HBEGF/EGFP)Aiwsk> |
Frozen Mouse Embryo | B6(FVB)-Mgl2<tm1.1(HBEGF/EGFP)Aiwsk>/J | $2595.00 |
Frozen Mouse Embryo | B6(FVB)-Mgl2<tm1.1(HBEGF/EGFP)Aiwsk>/J | $2595.00 |
Frozen Mouse Embryo | B6(FVB)-Mgl2<tm1.1(HBEGF/EGFP)Aiwsk>/J | $3373.50 |
Frozen Mouse Embryo | B6(FVB)-Mgl2<tm1.1(HBEGF/EGFP)Aiwsk>/J | $3373.50 |
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