These transgenic mice carry flag-tagged mouse Hist2h2be (histone cluster 2, H2be) downstream of the rat Omp (olfactory marker protein) promoter. High levels of FLAG-H2BE are seen in all mature olfactory neurons except for a subset in zone 2.
Catherine Dulac, Harvard University, HHMI
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Hist2h2be (histone cluster 2, H2be) is expressed exclusively in olfactory chemosensory neurons. Expression is reduced by sensory activity, and it alters gene expression and promotes neuronal cell death in mature olfactory sensory neurons, potentially modulating olfactory neuronal population dynamics to adapt the olfactory repertoire to the environment.
These transgenic mice carry flag-tagged mouse Hist2h2be (histone cluster 2, H2be) downstream of the rat Omp (olfactory marker protein) promoter. The flag reporter facilitates and characterization and unambiguous identification of Hist2h2be and distinguishes it from canonical H2B. Immunofluorescent examination in the main olfactory epithelium (MOE), reveals a 10 fold increase in nuclear expression in a pattern similar to that of endogenous OMP, with the exception of a small patch of neurons in the medial part of the MOE that lack expression. High levels of FLAG-H2BE are seen in all mature olfactory neurons except for a subset in zone 2. Transgenic mice display higher levels of apoptosis among olfactory sensory neurons in the MOE.
A transgenic vector carrying flag-tagged mouse Hist2h2be was placed downstream of the rat Omp (olfactory marker protein) promoter was introduced to C57BL/6J x DBA/2J (B6D2F1) embryos. Resultant founder line 14, carrying an estimated 12 copies of the transgene, was backcrossed to C57BL/6J for approximately 5 generations by the donating laboratory.
Expressed Gene | H2bc21, H2B clustered histone 21, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 14, Catherine Dulac |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | H2be-GF; Omp:Flag-H2be; Tg(Omp-Hist2h2be)#aDlc |
Gene Symbol and Name | Tg(Omp-Hist2h2be)14Dlc, transgene insertion 14, Catherine Dulac |
Gene Synonym(s) | |
Promoter | Omp, olfactory marker protein, mouse, laboratory |
Expressed Gene | H2bc21, H2B clustered histone 21, mouse, laboratory |
Strain of Origin | (C57BL/6J x DBA/2J)F1 |
Chromosome | UN |
Molecular Note | The promoter drives olfactory-specific expression of a FLAG-tagged coding sequence. Line 14 contains 12 copies of the transgene. |
Heterozygous and homozygous mice are viable and fertile.
When using the B6.Cg-Tg(Omp-Hist2h2be)14Dlc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023820 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Omp-Hist2h2be)14Dlc |
Frozen Mouse Embryo | B6.Cg-Tg(Omp-Hist2h2be)14Dlc/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Omp-Hist2h2be)14Dlc/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Omp-Hist2h2be)14Dlc/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Omp-Hist2h2be)14Dlc/J | $3373.50 |
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