These transgenic mice express the mouse Prg4 (proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) gene under the regulatory control of the cartilage-specific mouse Col2a1 (collagen, type II, alpha 1) promoter. Long-term expression does not adversely affect skeletal development but protects from developing signs of age-related and posttraumatic osteoarthritis (OA).
Brendan Lee, Baylor College of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Prg4 (proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)) plays a protective role against osteoarthritis (OA). The PRG4 protein provides synovial fluid with the ability to dissipate strain energy under load, protecting bones of the joint, and also inhibits transcriptional programs that promote cartilage catabolism and hypertrophy.
These transgenic mice express the mouse Prg4 under the regulatory control of the cartilage-specific mouse Col2a1 (collagen, type II, alpha 1) promoter. PRG4 is expressed ectopically in growth plate cartilage and overexpressed in articular cartilage throughout development and adulthood. Long-term expression does not adversely affect skeletal development but protects from developing signs of age-related OA. Protective effects are also seen in models of posttraumatic OA created by cruciate ligament transection. Wildtype FVB/N mice develop changes consistent with moderate OA by 10 months of age (OARSI grade 3.5), but Prg4 transgenic animals show less severe signs of OA at the same age (OARSI grade 2). Whereas wildtype mice show increased expression of Col10a1 and Mmp13 (markers of cartilage hypertrophy and degradation), they do not show a qualitative increases in non-calcified regions of articular cartilage in the transgenic mice. Wildtype mice show a decrease in articular cartilage volume as well as in the bone area covered by cartilage, but transgenic mice show preservation of articular cartilage volumes and surface area. This model suggests a new disease-altering therapy for osteoarthritis.
A transgenic vector incorporating wildtpe tyrosinase (Tyr+), the mouse Prg4 gene, the postranscriptional regulator element (PRE) element from the woodchuck hepatitis virus (WPRE), and polyA driven by the mouse Col2a1 promoter was introduced to FVB/NJ embryos via pronuclear microinjection. Animals in the colony at The Jackson Laboratory are albino with pink eyes. This strain was maintained on an FVB genetic background by the donating lab.
Expressed Gene | Prg4, proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein), mouse, laboratory |
---|---|
Site of Expression |
Allele Name | transgene insertion 1, Brendan Lee |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Col2a1-Prg4)1Brle, transgene insertion 1, Brendan Lee |
Gene Synonym(s) | |
Promoter | Col2a1, collagen, type II, alpha 1, mouse, laboratory |
Expressed Gene | Prg4, proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein), mouse, laboratory |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | A transgenic vector incorporating wild-type tyrosinase Tyr<+>, the mouse gene, postranscriptional regulator element (PRE) element from the woodchuck hepatitis virus (WPRE) and polyA driven by the cartilage-specific mouse Col2a1 promoter. Line 1 was generated. |
Hemizygotes and homozygotes are viable and fertile.
When using the FVB-Tg(Col2a1-Prg4)1Brle/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023763 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Col2a1-Prg4)1Brle |
Frozen Mouse Embryo | FVB-Tg(Col2a1-Prg4)1Brle/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Col2a1-Prg4)1Brle/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Col2a1-Prg4)1Brle/J | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Col2a1-Prg4)1Brle/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.