Overview

Also Known As: Slc17a7-IRES2-Cre or Vglut1-IRES2-Cre-D

Slc17a7-IRES2-Cre-D knockin mice have Cre recombinase expression directed to Vglut1-expressing cells, without disrupting endogenous vesicular glutamate transporter 1 expression. These mice may be useful for studying glutamatergic synaptic vesicle trafficking and vesicle-bound, sodium-dependent phosphate transportation.

Donating Investigator

Hongkui Zeng, Allen Institute for Brain Science

Genetic overview

Genetic Background	Generation
	N3F1+pN2F2 (2019-10-03 00:00:00)

Slc17a7^{tm1.1(cre)Hze}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Slc17a7	solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7

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Research Applications

Research Tools
Neurobiology Research
Cell Biology Research

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Base Price

Starting at:

$255.00 Domestic price for female

333.51 Domestic price for breeder pair

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Details

Detailed Description

The Slc17a7-IRES2-Cre-D (also called Slc17a7-IRES2-Cre-Δ or Slc17a7-IRES2-Cre-Δneo/hygro) targeted mutation has an IRES2 sequence and a Cre recombinase gene inserted downstream of the translational STOP codon of the vesicular glutamate transporter 1 gene (Slc17a7 or Vglut1). As such, Slc17a7-IRES2-Cre-D mice have both endogenous gene and Cre recombinase expression directed to Slc17a7-expressing cells by the endogenous promoter/enhancer elements of the vesicular glutamate transporter 1 locus. When Slc17a7-IRES2-Cre-D mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Slc17a7-expressing cells in the offspring.

The donating investigator reports Cre recombinase expression (in situ hybridization using a Cre-specific probe) is observed in a pattern very similar to that of the endogenous Slc17a7 gene. Specifically, cre expression is strong throughout the cortex, olfactory bulb and anterior olfactory nuclei. Scattered cre expression is seen in striatum and hippocampus. Cre recombinase is enriched in restricted populations in pons, superior colliculus and anterodorsal nucleus of thalamus. The donating investigator did not examine cre expression in tissues other than brain.

Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. The donating investigator has not attempted to generate homozygous mice to date (September 2013).

For characterization information, see images at the Allen Institute for Brain Science website (Slc17a7-IRES2-Cre images).

Development

These Slc17a7-IRES2-Cre-D mice (also called Slc17a7-IRES2-Cre-Δ or Slc17a7-IRES2-Cre-Δneo/hygro) have an IRES2 sequence and a Cre recombinase gene inserted immediately downstream of the translational STOP codon of the vesicular glutamate transporter 1 gene (Slc17a7 or Vglut1). The specific details are below.

The targeting vector contained, from 5' to 3',
a partial Slc17a7 sequence spanning exon 1 through intron 9,
an frt3 site within Slc17a7 intron 9,
a partial Slc17a7 exon 10 sequence up to and including the endogenous stop codon,
an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence),
a Cre recombinase gene,
a bovine growth hormone polyA sequence,
an AttB site,
a PGK promoter-Neomycin resistance gene-PGK polyA cassette,
an frt5 site,
an mRNA splice acceptor,
the 3' portion of the hygromycin gene with SV40 late polyA signal,
and an AttP site.

This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice (C57BL/6J congenic background; see Stock No. 007743) to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL).

The resulting Slc17a7-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice for several generations (and the PhiC31 transgene was removed). In 2013, males from generation N3F1 were sent to The Jackson Laboratory Repository. Upon arrival, males were used to cryopreserve sperm. To establish the living Slc17a7-IRES2-Cre-D mouse colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J inbred females (Stock No. 000664).

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	cre expression is strong throughout the cortex, olfactory bulb and anterior olfactory nuclei. Scattered cre expression is seen in striatum and hippocampus. Cre recombinase is enriched in restricted populations in pons, superior colliculus and anterodorsal nucleus of thalamus.

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Harris JA; Hirokawa KE; Sorensen SA; Gu H; Mills M; Ng LL; Bohn P; Mortrud M; Ouellette B; Kidney J; Smith KA; Dang C; Sunkin S; Bernard A; Oh SW; Madisen L; Zeng H. 2014. Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits 8:76PubMed: 25071457 MGI: J:220523

View All References

Genetics

Slc17a7^{tm1.1(cre)Hze}

Allele Symbol: Slc17a7^{tm1.1(cre)Hze}

Allele Name	targeted mutation 1.1, Hongkui Zeng
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	targeted mutation 1.1, Hongkui Zeng; Slc17a7^{tm1.1(cre)Hze}
Gene Symbol and Name	Slc17a7, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Gene Synonym(s)	BNPI; VGLUT1; RIKEN cDNA 2900052E22 gene; AI851913; 2900052E22Rik; expressed sequence AI851913; 2900052E22Rik; Vglut1
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	cre expression is strong throughout the cortex, olfactory bulb and anterior olfactory nuclei. Scattered cre expression is seen in striatum and hippocampus. Cre recombinase is enriched in restricted populations in pons, superior colliculus and anterodorsal nucleus of thalamus.
Strain of Origin	(129S6/SvEvTac x C57BL/6NCrl)F1
Chromosome	7
Molecular Note	The targeting vector contained, from 5' to 3', a partial Slc17a7 sequence spanning exon 1 through intron 9, an frt3 site within Slc17a7 intron 9, a partial Slc17a7 exon 10 sequence including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence , a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). The resulting Slc17a7-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice for several generations to remove PhiC31 transgene.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis: Cre-lox System
- Cre-lox System
  - Cre Recombinase Expression
- Developmental Biology Research
  - Cre-lox System
- Neurobiology Research
  - cell marker
Neurobiology Research
- Neurotransmitter Receptor and Synaptic Vesicle Defects
- Channel and Transporter Defects
  - calcium: glutamate receptor
- Cre-lox System
  - Cre Recombinase expression in neural tissue
Cell Biology Research
- Channel and Transporter Defects
- Vesicular Trafficking

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Slc17a7^{tm1.1(cre)Hze}/Slc17a7⁺
involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NCr

normal phenotype

no abnormal phenotype detected
- mice are viable and fertile with no gross physical or behavioral abnormalities

References

Harris JA; Hirokawa KE; Sorensen SA; Gu H; Mills M; Ng LL; Bohn P; Mortrud M; Ouellette B; Kidney J; Smith KA; Dang C; Sunkin S; Bernard A; Oh SW; Madisen L; Zeng H. 2014. Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits 8:76PubMed: 25071457 MGI: J:220523

Additional - Slc17a7^{tm1.1(cre)Hze} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated PCR:Slc17a7^{tm1.1(cre)Hze}
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred to wildtype siblings or to C57BL/6J inbred mice (Stock No. 000664). The donating investigator has not attempted to generate homozygous mice to date (September 2013).
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x Wild-type
- Wild-type x Heterozygote
Citation
When using the Slc17a7-IRES2-Cre or Vglut1-IRES2-Cre-D mouse strain in a publication, please cite the originating article(s) and include JAX stock #023527 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

FGB29 (Standard)

Pricing & Availability

Available

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International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Not-For-Profit & Academic Pricing

Service	Genotype	Price
	Heterozygous or Wildtype for Slc17a7<tm1.1(cre)Hze>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6;129S-Slc17a7<tm1.1(cre)Hze>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129S-Slc17a7<tm1.1(cre)Hze>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129S-Slc17a7<tm1.1(cre)Hze>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6;129S-Slc17a7<tm1.1(cre)Hze>/J Frozen Embryo	$3373.50

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Also Known As: Slc17a7-IRES2-Cre or Vglut1-IRES2-Cre-D

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Slc17a7tm1.1(cre)Hze

Allele Symbol: Slc17a7tm1.1(cre)Hze

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Slc17a7tm1.1(cre)Hze/Slc17a7+involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NCr

normal phenotype

References

Additional - Slc17a7tm1.1(cre)Hze related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Slc17a7^{tm1.1(cre)Hze}

Allele Symbol: Slc17a7^{tm1.1(cre)Hze}

Genotype: Slc17a7^{tm1.1(cre)Hze}/Slc17a7⁺
involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NCr

Additional - Slc17a7^{tm1.1(cre)Hze} related