In this strain, a neo cassette replaces exon 1 of the Scp2 (sterol carrier protein 2, liver) gene, generating a null SCP-x allele, but allowing expression of SCP2. Homozygous mice exhibit gender-dependent alterations in serum and liver lipid profiles and diet-induced (0.5% phytol) weight loss, enlarged livers, liver necrosis and inflammation. This strain may be useful for studying lipid and glucose metabolism and peroxisome-associated disorders.
Ann Kier, Texas A&M University, College of Veterinary Medicine and Biomedcial Sciences
A targeting vector containing neomycin resistance gene was used to replace exon 1. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to crossed to C57BL/6NCr and offspring were backcrossed to C57BL/6NCr for 12 generations. Upon arrival, mice were bred to C57BL/6NJ for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Ann B Kier|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Scp2, sterol carrier protein 2, liver|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exon 1 was replaced with a neomycin resistance cassette. Absence of the protein was confirmed by Western analysis.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6N.129P2-Scp2tm1Kier/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36977 in your Materials and Methods section.