These pBi-Noggin-eGFP transgenic mice allow Tet-inducible inhibition of transforming growth factor (TGF)-beta signal transduction (including bone morphogenetic protein (BMP)-signaling pathway), and may be useful in studying these signaling pathways during embryonic development, in postnatal tissues, and in sites of adult neurogenesis. These animals may also be used for mobilizing endogenous neural stem cell populations to replace neurons and oligodendrocytes lost as a result of disease, injury, and normal or pathological aging.
K. Sue O'Shea, University of Michigan Medical School
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter, Inducible, Inserted expressed sequence) |
The pBi-Noggin-eGFP transgene has a bidirectional tetracycline-inducible promoter controlling expression of noggin and eGFP. No transgenic noggin or eGFP expression is reported in the absence of tetracycline-controlled transactivator protein (tTA). When bred to other mice expressing tTA or reverse tetracycline-controlled transactivator protein (rtTA), simultaneous noggin and eGFP expression in the resulting double mutant offspring can be regulated by tetracycline or its analog doxycycline (dox).
Mice from founder line 53 were found to have the highest and most stable expression of transgenic noggin and eGFP after dox administration, with the lowest background expression levels. When induced, eGFP expression is readily observed via immunohistochemical detection, but direct eGFP fluorescence is very faint. FVB.pBi-Noggin-eGFP line 53 hemizygotes are viable and fertile, with no reported phenotypic abnormalities. The donating investigator reports the transgene copy number and genomic integration site of the transgene is unknown. The donating investigator also reports that homozygous mice are viable and fertile, with behavior and gene expression the same as hemizygous animals.
When FVB.pBi-Noggin-eGFP line 53 mice are bred to mice expressing rtTA in central nervous system (Nestin-rtTA; see Stock No. 004127), the resulting double mutant offspring may be useful in mobilizing endogenous neural stem cell populations to replace neurons and oligodendrocytes lost as a result of disease, injury, and normal or pathological aging. Immunohistochemical localization of nestin and eGFP in sections of doxycycline-induced brain also showed complete overlap. In the absence of doxycycline, noggin over-expression is not observed.
The pBi-Noggin-eGFP transgene was created by Dr. K. Sue O'Shea (University of Michigan, Ann Arbor) to have the Pbi-1 bi-directional tetracycline-responsive promoter with an enhanced green fluorescent protein coding region (eGFP) on one side, and a mouse noggin cDNA sequence (encompassing the complete coding region) on the other side. The Pbi-1 promoter contains the Tet-responsive element (TRE or tetO7; seven copies of the 42-bp tet operator sequence [tetO]) placed between two constitutively active, minimal human cytomegalovirus promoters (PminCMV). The eGFP is followed by a beta-globin polyA signal, and the noggin cDNA is followed by an SV40 polyA signal. The ~5 kbp pBi-Noggin-eGFP transgene was microinjected into oocytes from FVB.Nestin-rtTA (Stock No. 004127) female mice fertilized by FVB/N males. Mice from founder line 53 were found to have the highest and most stable expression of transgenic noggin and eGFP after dox administration, with the lowest background expression levels.
Next, animals were bred together (and the Nestin-rtTA transgene was removed). In 2015, FVB.pBi-Noggin-eGFP males were sent to The Jackson Laboratory Repository. Upon arrival, males were used to cryopreserve sperm. To establish the living FVB.pBi-Noggin-eGFP mouse colony, an aliquot of the frozen sperm was used to fertilize oocytes from FVB/NJ inbred females (Stock No. 001800).
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Expressed Gene | Nog, noggin, mouse, laboratory |
Site of Expression | Simultaneous Nog and EGFP expression can be regulated with tetracycline when combined with a mutant carrying tTA or rtTA. |
Allele Name | transgene insertion 53, K Sue O'Shea |
---|---|
Allele Type | Transgenic (Reporter, Inducible, Inserted expressed sequence) |
Allele Synonym(s) | pBi-Noggin-EGFP |
Gene Symbol and Name | Tg(tetO-Nog,-EGFP)53Hda, transgene insertion 53, K Sue O'Shea |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Nog, noggin, mouse, laboratory |
Site of Expression | Simultaneous Nog and EGFP expression can be regulated with tetracycline when combined with a mutant carrying tTA or rtTA. |
Strain of Origin | FVB/N-Tg(Nes-rtTA)306Rvs/J |
Chromosome | UN |
Molecular Note | The transgene has a Pbi-1 bi-directional tetracycline-responsive promoter with an enhanced green fluorescent protein coding region (eGFP) on one side, and a mouse noggin cDNA sequence (encompassing the complete coding region) on the other side. The promoter contains the Tet-responsive element (TRE) placed between two constitutively active, minimal human cytomegalovirus promoters. The eGFP is followed by a beta-globin polyA signal, and the noggin cDNA is followed by an SV40 polyA signal. |
When maintaining a live colony, hemizygous mice may be bred with wildtype (noncarrier) mice from the colony and/or with FVB/NJ inbred mice (Stock No. 001800). The donating investigator also reports that homozygous mice are viable and fertile, with behavior and gene expression the same as hemizygous animals.
When using the pBi-Noggin-eGFP line 53 mouse strain in a publication, please cite the originating article(s) and include JAX stock #023410 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(tetO-Nog,-EGFP)53Hda |
Frozen Mouse Embryo | FVB/N-Tg(tetO-Nog -EGFP)53Hda/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(tetO-Nog -EGFP)53Hda/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(tetO-Nog -EGFP)53Hda/J | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(tetO-Nog -EGFP)53Hda/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.