These BAC transgenic mice express Cre recombinase under control of the Itgax gene (or Cd11c) promoter/enhancer regions within the BAC transgene. These Cd11c-Cre mice are suitable for use in the deletion of floxed sequences in CD8-, CD8+ dendritic cells, tissue-derived dendritic cells from lymph nodes, lung and epidermis, as well as plasmacytoid dendritic cells.
Pere Santamaria, University of Calgary
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Cd11c-Cre transgenic mice express Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter. Cre-mediated recombination is detected in more than 95% of conventional CD11chigh dendritic cells both from lymphoid organs and from non-lymphoid tissues such as lung and epidermis, and in 50-80% of plasmacytoid dendritic cells. The dendritic cell compartment of transgenic mice is normal. Relatively low amounts of recombination are detected in lymphocytes (<10%), NK cells (12%) and myeloid cells (<1%). No increase of recombination frequency is observed in CD11clow-activated T cells.
Mice hemizygous for the transgene are viable and fertile.
These NOD.Cd11c-cre transgenic mice (as well as B6.CD11c-cre transgenic mice [Stock No. 008068]) are an effective tool for generating tissue-specific targeted mutants for studying dendritic cell homeostasis and function.
The Itgax-cre (Cd11c-Cre) BAC transgene was designed in the laboratory of Dr. Boris Reizis (Columbia University Medical Center). The mouse bacterial artificial chromosome (BAC) RP24-361C4, containing the integrin alpha X gene (Itgax or Cd11c) but lacking the 5 prime end of the adjacent Itgam gene, was obtained. A Cre recombinase and a polyA signal sequence was introduced into the first exon of Itgax on the RP24-361C4 BAC via homologous recombination/BAC recombineering. The resulting ~160 kbp transgenic construct was introduced into B6CBAF1/J donor eggs. Founder line 1-1 was consequently established. The mice were crossed to 129S6/SvEvTac for several generations, and then backcrossed to C57BL/6 for more than 12 generations. The resulting C57BL/6-congenic strain (B6.Cd11c-cre) was sent to The Jackson Laboratory Repository in 2007 as Stock No. 008068. Dr. Pere Santamaria (University of Calgary) obtained some B6.Cd11c-cre mice and subsequently backcrossed them to NOD/ShiLtJ mice for at least ten generations. The resulting NOD/ShiLtJ-congenic strain (NOD.Cd11c-cre) was sent to The Jackson Laboratory Repository in 2013 as Stock No. 023203. Upon arrival, NOD.Cd11c-cre was crossed to NOD/ShiLtJ mice (Stock No. 001976) for at least one generation to establish the living colony.
2014 SNP data indicates that the transgene may have integrated on Chromosome 2.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | most conventional CD11chigh dendritic cells and many plasmacytoid dendritic cells; low expression in lymphocytes, NK cells, and myeloid cells |
Allele Name | transgene insertion 1-1, Boris Reizis |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | CD11cCre; Cd11c-cre; CD11cCreT |
Gene Symbol and Name | Tg(Itgax-cre)1-1Reiz, transgene insertion 1-1, Boris Reizis |
Gene Synonym(s) | |
Promoter | Itgax, integrin alpha X, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | most conventional CD11chigh dendritic cells and many plasmacytoid dendritic cells; low expression in lymphocytes, NK cells, and myeloid cells |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | A transgenic construct containing sequence encoding cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter, and a bovine growth hormone poly A signal sequence, was introduced into B6CBAF1/J donor eggs. BAC clone RP24-361C4 (BACPAC Resources) containing the Itgax (Cd11c) gene but lacking the 5' end of the adjacent Itgam gene, was modified by ET recombination to generated the transgene construct. Founder line 1-1 was consequently established. Cre recombinase expression is detected in CD8-, CD8+ dendritic cells, tissue derived dendritic cells from lymph nodes, lung and epidermis and plasmacytoid dendritic cells. Background recombination is detected in lymphocytes (<10%) and myeloid cells (<1%). |
Mutations Made By | Boris Reizis, Columbia University Medical Center |
When maintaining a live colony, these mice can be bred as hemizygotes.
When using the Cd11c-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #023203 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizyous or Non carrier for Tg(Itgax-cre)1-1Reiz |
Frozen Mouse Embryo | NOD.Cg-Tg(Itgax-cre)1-1Reiz/PesaJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tg(Itgax-cre)1-1Reiz/PesaJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tg(Itgax-cre)1-1Reiz/PesaJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-Tg(Itgax-cre)1-1Reiz/PesaJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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