NOD.Cg-Tg(Itgax-cre)1-1Reiz/PesaJ

Stock number: 023203
Product name: Cd11c-Cre
Strain synonyms: CD11cCre^T
Research areas: Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These BAC transgenic mice express Cre recombinase under control of the Itgax gene (or Cd11c) promoter/enhancer regions within the BAC transgene. These Cd11c-Cre mice are suitable for use in the deletion of floxed sequences in CD8^-, CD8⁺ dendritic cells, tissue-derived dendritic cells from lymph nodes, lung and epidermis, as well as plasmacytoid dendritic cells.

Detailed description

Cd11c-Cre transgenic mice express Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter. Cre-mediated recombination is detected in more than 95% of conventional CD11c^high dendritic cells both from lymphoid organs and from non-lymphoid tissues such as lung and epidermis, and in 50-80% of plasmacytoid dendritic cells. The dendritic cell compartment of transgenic mice is normal. Relatively low amounts of recombination are detected in lymphocytes (<10%), NK cells (12%) and myeloid cells (<1%). No increase of recombination frequency is observed in CD11c^low-activated T cells. Mice hemizygous for the transgene are viable and fertile. These NOD.Cd11c-cre transgenic mice (as well as B6.CD11c-cre transgenic mice [Stock No. 008068]) are an effective tool for generating tissue-specific targeted mutants for studying dendritic cell homeostasis and function.

Development

The Itgax-cre (Cd11c-Cre) BAC transgene was designed in the laboratory of Dr. Boris Reizis (Columbia University Medical Center). The mouse bacterial artificial chromosome (BAC) RP24-361C4, containing the integrin alpha X gene (Itgax or Cd11c) but lacking the 5 prime end of the adjacent Itgam gene, was obtained. A Cre recombinase and a polyA signal sequence was introduced into the first exon of Itgax on the RP24-361C4 BAC via homologous recombination/BAC recombineering. The resulting ~160 kbp transgenic construct was introduced into B6CBAF1/J donor eggs. Founder line 1-1 was consequently established. The mice were crossed to 129S6/SvEvTac for several generations, and then backcrossed to C57BL/6 for more than 12 generations. The resulting C57BL/6-congenic strain (B6.Cd11c-cre) was sent to The Jackson Laboratory Repository in 2007 as Stock No. 008068. Dr. Pere Santamaria (University of Calgary) obtained some B6.Cd11c-cre mice and subsequently backcrossed them to NOD/ShiLtJ mice for at least ten generations. The resulting NOD/ShiLtJ-congenic strain (NOD.Cd11c-cre) was sent to The Jackson Laboratory Repository in 2013 as Stock No. 023203. Upon arrival, NOD.Cd11c-cre was crossed to NOD/ShiLtJ mice (Stock No. 001976) for at least one generation to establish the living colony.

2014 SNP data indicates that the transgene may have integrated on Chromosome 2.

Allele

Symbol: Tg(Itgax-cre)1-1Reiz
Name: transgene insertion 1-1, Boris Reizis
MGI accession ID: MGI:3763248
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Itgax-cre)1-1Reiz
Marker name: transgene insertion 1-1, Boris Reizis
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: most conventional CD11c^high dendritic cells and many plasmacytoid dendritic cells; low expression in lymphocytes, NK cells, and myeloid cells
Molecular note: A transgenic construct containing sequence encoding cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter, and a bovine growth hormone poly A signal sequence, was introduced into B6CBAF1/J donor eggs. BAC clone RP24-361C4 (BACPAC Resources) containing the Itgax (Cd11c) gene but lacking the 5' end of the adjacent Itgam gene, was modified by ET recombination to generated the transgene construct. Founder line 1-1 was consequently established. Cre recombinase expression is detected in CD8-, CD8+ dendritic cells, tissue derived dendritic cells from lymph nodes, lung and epidermis and plasmacytoid dendritic cells. Background recombination is detected in lymphocytes (<10%) and myeloid cells (<1%).