The Interstitial retinal binding protein 3 is a large glycoprotein that binds retinoids and fatty acids, and transports retinoids between the retinal pigment epithelium and the photoreceptors, mediating cone pigment regeneration. These mice carry a targeted mutation for the Rbp3 gene in which the promoter and most of exon 1 has been replaced by a NEO selection cassette. Mice that are homozygous for the targeted mutation are viable and fertile. A truncated transcript product is detected by Northern blot analysis of total retinal RNA from homozygous animals, however no protein gene product is detected by immunocytochemical or Western blot analysis. Homozygotes exhibit myopia, and progressive retinal degeneration is detected at postnatal day 11. The onset of ocular enlargement occurs between postnatal day 7 and 10. Retinal outer nuclear layer is approximately 40% and electoretinographic (ERG) response is reduced by approximately 40% when compared to wildtype controls.
A targeting vector containing a PGK-NEO cassette was used to disrupt approximately 4.4kb of sequence, including the promoter and most of exon 1. The construct was electroporated into unspecified 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were bred to C57BL/6J to achieve germline transmission. The mice were backcrossed to C57BL/6J for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Gregory I Liou|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Rbp3, retinol binding protein 3, interstitial|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Approximately 4.4kb of sequence was replaced by a PGK-neo selection cassette. The deleted region contained the promoter and all but 24 base pairs of exon 1. While transcript was undetected by RT-PCR analysis in homozygous mutant mice, Northern blot analysis of total retinal RNA showed the presence of a truncated transcript containing the PGK-neo transgene and the untargeted exons. Immunocytochemistry showed the absence of protein in homozygous mutant mice. Western blot analysis confirmed the staining results as well as verified the lack of protein produced from the truncated transcript.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129P2-Rbp3tm1Gil/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023080 in your Materials and Methods section.