Krt12-Cre mice expresses Cre recombinase from endogenous Krt12 promoter/enhancer elements. This strain represents an effective tool for generating corneal epithelia specific-targeted mutants.
Winston W.-Y. Kao, University of Cincinnati
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Krt12 | keratin 12 |
Krt12-Cre mice express Cre recombinase from endogenous Keratin 12 (Krt12) promoter/enhancer elements. Homozygous mice are viable and fertile. When Foxd1 is induced, cre is expressed specifically in corneal epithelia. When these mice are bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in corneal epithelia cells of the offspring.
A targeting vector was designed to insert an internal ribosome entry site (IRES) fused to a nuclear localization signal and a Cre recombinase coding sequence (GC), followed by a neomycin resistance cassette, between the stop codon and the polyadenylation signal of the Keratin 12 (Krt12) gene. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with Black Swiss females. These Krt12-Cre mice were bred to C57BL/6J mice for at least 20 generations. Upon arrival at The Jackson Laboratory, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre-mediated recombination will result in deletion of the floxed sequences in corneal epithelia cells of the offspring. |
Allele Name | targeted mutation 3, Winston W Y Kao |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | K12Cre; Krt12Cre; Krt12-Cre |
Gene Symbol and Name | Krt12, keratin 12 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre-mediated recombination will result in deletion of the floxed sequences in corneal epithelia cells of the offspring. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | An IRES-cre minigene was inserted into the site immediately behind the stop codon in exon 8. The mutant allele encodes a bicistronic mRNA with cre recombinase being under the control of the endogenous regulatory sequences. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129(Cg)-Krt12tm3(cre)Wwk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023055 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Krt12<tm3(cre)Wwk> |
Frozen Mouse Embryo | B6.129(Cg)-Krt12<tm3(cre)Wwk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Krt12<tm3(cre)Wwk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Krt12<tm3(cre)Wwk>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Krt12<tm3(cre)Wwk>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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