B6.129(Cg)-Krt12^tm3(cre)Wwk/J

Stock number: 023055
Research areas: Cell Biology Research, Developmental Biology Research, Research Tools, Sensorineural Research

Description

Krt12-Cre mice expresses Cre recombinase from endogenous Krt12 promoter/enhancer elements. This strain represents an effective tool for generating corneal epithelia specific-targeted mutants.

Detailed description

Krt12-Cre mice express Cre recombinase from endogenous Keratin 12 (Krt12) promoter/enhancer elements. Homozygous mice are viable and fertile. When Foxd1 is induced, cre is expressed specifically in corneal epithelia. When these mice are bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in corneal epithelia cells of the offspring.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES) fused to a nuclear localization signal and a Cre recombinase coding sequence (GC), followed by a neomycin resistance cassette, between the stop codon and the polyadenylation signal of the Keratin 12 (Krt12) gene. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with Black Swiss females. These Krt12-Cre mice were bred to C57BL/6J mice for at least 20 generations. Upon arrival at The Jackson Laboratory, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Krt12^tm3(cre)Wwk
Name: targeted mutation 3, Winston W Y Kao
MGI accession ID: MGI:3624816
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Krt12
Marker name: keratin 12
Chromosome: 11
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre-mediated recombination will result in deletion of the floxed sequences in corneal epithelia cells of the offspring.
Molecular note: An IRES-cre minigene was inserted into the site immediately behind the stop codon in exon 8. The mutant allele encodes a bicistronic mRNA with cre recombinase being under the control of the endogenous regulatory sequences.